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验证数据展示

  • WB analysis of hTERT-RPE1 using 68331-1-Ig

    WB analysis of hTERT-RPE1 using 68331-1-Ig

    hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 68331-1-Ig (ZNHIT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 68331-1-Ig

    IF Staining of HeLa using 68331-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ZNHIT3 antibody (68331-1-Ig, Clone: 1C7A3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

ZNHIT3 Monoclonal antibody

ZNHIT3 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68331-1-Ig

产品说明书

CloneNo. 1C7A3

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

HNF 4a coactivator, TR interacting protein 3, TRIP 3, TRIP3, zinc finger, HIT type 3, ZNHIT3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhTERT-RPE1 cells, Jurkat cells, T-47D cells, MOLT-4 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68331-1-Ig targets ZNHIT3 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 ZNHIT3 fusion protein Ag29872 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 zinc finger, HIT type 3
别名 HNF 4a coactivator, TR interacting protein 3, TRIP 3, TRIP3, zinc finger, HIT type 3, ZNHIT3
计算分子量 155 aa, 18 kDa
观测分子量18 kDa
GenBank蛋白编号BC017931
基因名称 ZNHIT3
Gene ID (NCBI) 9326
RRIDAB_2935403
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15649
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZNHIT3, also named TRIP3, belongs to the zinc finger HIT (Zf-HIT) domain-containing proteins family. ZNHIT3 encodes a nuclear zinc finger protein previously implicated in transcriptional regulation and small nucleolar ribonucleoprotein particle assembly and thus possibly to pre-ribosomal RNA processing (PMID: 28335020). ZNHIT3 contains at least two domains: a ZN-HIT domain that consists of a double zinc-finger, probably involved in protein−protein interaction, and a PAC-HIT domain that folds into a clamp able to trap an α-helix, here again of about 20 residues, located in the sequence of NUFIP1 (PMID: 35315277). ZNHIT3 has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for ZNHIT3 antibody 68331-1-IgDownload protocol
IF protocol for ZNHIT3 antibody 68331-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols