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验证数据展示

  • WB analysis of hTERT-RPE1 using 68331-1-Ig (same clone as 68331-1-PBS)

    WB analysis of hTERT-RPE1 using 68331-1-Ig (same clone as 68331-1-PBS)

    hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 68331-1-Ig (ZNHIT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68331-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68331-1-Ig (same clone as 68331-1-PBS)

    IF Staining of HeLa using 68331-1-Ig (same clone as 68331-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ZNHIT3 antibody (68331-1-Ig, Clone: 1C7A3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68331-1-PBS in a different storage buffer formulation.

ZNHIT3 Monoclonal antibody, PBS Only

ZNHIT3 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68331-1-PBS

产品说明书

CloneNo. 1C7A3

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

WB, IF/ICC, Indirect ELISA

别名

HNF 4a coactivator, TR interacting protein 3, TRIP 3, TRIP3, zinc finger, HIT type 3, ZNHIT3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68331-1-PBS targets ZNHIT3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human
免疫原 ZNHIT3 fusion protein Ag29872 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 zinc finger, HIT type 3
别名 HNF 4a coactivator, TR interacting protein 3, TRIP 3, TRIP3, zinc finger, HIT type 3, ZNHIT3
计算分子量 155 aa, 18 kDa
观测分子量18 kDa
GenBank蛋白编号BC017931
基因名称 ZNHIT3
Gene ID (NCBI) 9326
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15649
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ZNHIT3, also named TRIP3, belongs to the zinc finger HIT (Zf-HIT) domain-containing proteins family. ZNHIT3 encodes a nuclear zinc finger protein previously implicated in transcriptional regulation and small nucleolar ribonucleoprotein particle assembly and thus possibly to pre-ribosomal RNA processing (PMID: 28335020). ZNHIT3 contains at least two domains: a ZN-HIT domain that consists of a double zinc-finger, probably involved in protein−protein interaction, and a PAC-HIT domain that folds into a clamp able to trap an α-helix, here again of about 20 residues, located in the sequence of NUFIP1 (PMID: 35315277). ZNHIT3 has 2 isoforms produced by alternative splicing.