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ZNHIT3 Monoclonal antibody, PBS Only

ZNHIT3 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C7A3

Cat no : 68331-1-PBS

Print datasheet

Synonyms

HNF 4a coactivator, TR interacting protein 3, TRIP 3, TRIP3, zinc finger, HIT type 3, ZNHIT3

验证数据展示

  • WB analysis of hTERT-RPE1 using 68331-1-Ig (same clone as 68331-1-PBS)

    WB analysis of hTERT-RPE1 using 68331-1-Ig (same clone as 68331-1-PBS)

    hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 68331-1-Ig (ZNHIT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68331-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68331-1-Ig (same clone as 68331-1-PBS)

    IF Staining of HeLa using 68331-1-Ig (same clone as 68331-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ZNHIT3 antibody (68331-1-Ig, Clone: 1C7A3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68331-1-PBS in a different storage buffer formulation.

产品信息

68331-1-PBS targets ZNHIT3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen ZNHIT3 fusion protein Ag29872 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name zinc finger, HIT type 3
Synonyms HNF 4a coactivator, TR interacting protein 3, TRIP 3, TRIP3, zinc finger, HIT type 3, ZNHIT3
Calculated Molecular Weight 155 aa, 18 kDa
Observed Molecular Weight18 kDa
GenBank Accession NumberBC017931
Gene Symbol ZNHIT3
Gene ID (NCBI) 9326
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15649
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ZNHIT3, also named TRIP3, belongs to the zinc finger HIT (Zf-HIT) domain-containing proteins family. ZNHIT3 encodes a nuclear zinc finger protein previously implicated in transcriptional regulation and small nucleolar ribonucleoprotein particle assembly and thus possibly to pre-ribosomal RNA processing (PMID: 28335020). ZNHIT3 contains at least two domains: a ZN-HIT domain that consists of a double zinc-finger, probably involved in protein−protein interaction, and a PAC-HIT domain that folds into a clamp able to trap an α-helix, here again of about 20 residues, located in the sequence of NUFIP1 (PMID: 35315277). ZNHIT3 has 2 isoforms produced by alternative splicing.