验证数据展示
经过测试的应用
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse ovary tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21279-1-AP targets ZP3 in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, IF-P, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, hamster |
| 免疫原 | ZP3 fusion protein Ag15791 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | zona pellucida glycoprotein 3 (sperm receptor) |
| 别名 | ZP3A/ZP3B, ZP3A, Zp-3, Zp 3, Zona pellucida sperm-binding protein 3 |
| 计算分子量 | 373 aa, 41 kDa |
| 观测分子量 | 47 kDa, 60 kDa |
| GenBank蛋白编号 | BC113949 |
| 基因名称 | ZP3 |
| Gene ID (NCBI) | 7784 |
| RRID | AB_11124319 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P21754 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Zona pellucida (ZP) is an extracellular matrix that surrounds the oocyte and early embryo, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. Mammalian ZP consists of three to four glycoproteins, called ZP1, ZP2, ZP3, ZP4. ZP3 (zona pellucida glycoprotein 3, also known as zona pellucida sperm-binding protein 3) is essential for sperm binding and zona matrix formation. Human ZP3 gene encodes predicted precursor protein of 47 kDa that is processed into mature glycoprotein with larger apparent molecular mass (PMID: 8588935; 10341000).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ZP3 antibody 21279-1-AP | Download protocol |
| IF protocol for ZP3 antibody 21279-1-AP | Download protocol |
| IP protocol for ZP3 antibody 21279-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Differ Transglutaminase 2 crosslinks zona pellucida glycoprotein 3 to prevent polyspermy. | ||
Am J Hum Genet A Recurrent Missense Mutation in ZP3 Causes Empty Follicle Syndrome and Female Infertility. | ||
Fertil Steril The critical role of ZP genes in female infertility characterized by empty follicle syndrome and oocyte degeneration. | ||
Hum Reprod Identification of a heterozygous variant of ZP2 as a novel cause of empty follicle syndrome in humans and mice. | ||
Cell Discov Abnormal gametogenesis induced by p53 deficiency promotes tumor progression and drug resistance. | ||
Hum Mutat A novel gene mutation in ZP3 loop region identified in patients with empty follicle syndrome |
