验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse ovary tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-21279 targets ZP3 in IF-P applications and shows reactivity with human, mouse samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | ZP3 fusion protein Ag15791 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | zona pellucida glycoprotein 3 (sperm receptor) |
| 别名 | Sperm receptor, Zona pellucida glycoprotein 3, Zona pellucida protein C, Zp 3, ZP3, ZP3A, ZP3A/ZP3B, ZP3B, ZPC |
| 计算分子量 | 373 aa, 41 kDa |
| 观测分子量 | 47 kDa, 60 kDa |
| GenBank蛋白编号 | BC113949 |
| 基因名称 | ZP3 |
| Gene ID (NCBI) | 7784 |
| RRID | AB_2934414 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P21754 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Zona pellucida (ZP) is an extracellular matrix that surrounds the oocyte and early embryo, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. Mammalian ZP consists of three to four glycoproteins, called ZP1, ZP2, ZP3, ZP4. ZP3 (zona pellucida glycoprotein 3, also known as zona pellucida sperm-binding protein 3) is essential for sperm binding and zona matrix formation. Human ZP3 gene encodes predicted precursor protein of 47 kDa that are processed into mature glycoprotein with larger apparent molecular mass (PMID: 8588935; 10341000).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ZP3 antibody CL488-21279 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |