Band 3/AE 1 Monoclonal antibody

Band 3/AE 1 Monoclonal Antibody for IHC, IF-P, ELISA
Cat No. 66984-1-Ig

产品说明书

CloneNo. 1E5C1

宿主/亚型

Mouse / IgG1

种属反应性

human and More (1)

应用

IHC, IF-P, ELISA

band 3, band 3/ AE1, SLC4A1, Anion exchanger 1, Anion exchange protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman placenta tissue
Planning an IHC experiment? We recommend our IHCeasy Band 3/AE1 Ready-To-Use IHC Kit. Band 3/AE1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

66984-1-Ig targets Band 3/AE 1 in IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF-P, ELISA Application Description
文献引用应用IHC
经测试反应性 human
文献引用反应性mouse
免疫原 Band 3/AE 1 fusion protein Ag28058 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group)
别名 band 3, band 3/ AE1, SLC4A1, Anion exchanger 1, Anion exchange protein 1
计算分子量 911 aa, 102 kDa
GenBank蛋白编号BC101570
基因名称 band 3
Gene ID (NCBI) 6521
RRIDAB_2918479
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP02730
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Anion exchanger 1 (AE1), also known as band 3, is a 95-100 kDa transmembrane glycoprotein which is abundantly expressed in erythrocyte plasma membrane and mediates the electroneutral exchange of Cl- and HCO3-. It contains a 52-55 kDa C-terminal membrane crossing domain and a 43 kDa N-terminal cytoplasmic domain. Degradation of band 3 protein occurred during erythrocyte aging. The oxidative stress can also activate the proteolysis of band 3 through caspases. This antibody is expected to recognize the intact band 3 (95-100 kDa) and various degradation products (38-60 kDa). (PMID: 22890269, 29240292, 28130418)

实验方案

Product Specific Protocols
IHC protocol for Band 3/AE 1 antibody 66984-1-IgDownload protocol
IF protocol for Band 3/AE 1 antibody 66984-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Neuron

Single-cell dissection of the human blood-brain barrier and glioma blood-tumor barrier

Authors - Yuan Xie
Loading...