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验证数据展示

  • IF Staining of human placenta using CL488-66984

    IF Staining of human placenta using CL488-66984

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CoraLite® Plus 488 band 3/ AE1 antibody (CL488-66984, Clone: 1E5C1 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated Band 3/AE 1 Monoclonal antibody

Band 3/AE 1 Monoclonal Antibody for IF-P
Cat No. CL488-66984

Print datasheet

CloneNo. 1E5C1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF-P

Synonyms

band 3, band 3/ AE1, SLC4A1, Anion exchanger 1, Anion exchange protein 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman placenta tissue
Planning an IHC experiment? We recommend our IHCeasy Band 3/AE1 Ready-To-Use IHC Kit. Band 3/AE1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66984-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66984 targets Band 3/AE 1 in IF-P applications and shows reactivity with human samples.

Tested Applications IF-P Application Description
Tested Reactivity human
Immunogen Band 3/AE 1 fusion protein Ag28058 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group)
Synonyms band 3, band 3/ AE1, SLC4A1, Anion exchanger 1, Anion exchange protein 1
Calculated Molecular Weight 911 aa, 102 kDa
GenBank Accession NumberBC101570
Gene Symbol band 3
Gene ID (NCBI) 6521
RRIDAB_2934508
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP02730
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Anion exchanger 1 (AE1), also known as band 3, is a 95-100 kDa transmembrane glycoprotein which is abundantly expressed in erythrocyte plasma membrane and mediates the electroneutral exchange of Cl- and HCO3-. It contains a 52-55 kDa C-terminal membrane crossing domain and a 43 kDa N-terminal cytoplasmic domain. Degradation of band 3 protein occurred during erythrocyte aging. The oxidative stress can also activate the proteolysis of band 3 through caspases. This antibody is expected to recognize the intact band 3 (95-100 kDa) and various degradation products (38-60 kDa). (PMID: 22890269, 29240292, 28130418)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Band 3/AE 1 antibody CL488-66984Download protocol
Standard Protocols
Click here to view our Standard Protocols