验证数据展示
经过测试的应用
Positive WB detected in | Raji cells, mouse brain tissue, A431 cells |
Planning an IHC experiment? We recommend our IHCeasy CBL Ready-To-Use IHC Kit. CBL primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66576-1-Ig
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL750-66576 targets c-Cbl in WB applications and shows reactivity with Human, mouse samples.
经测试应用 | WB Application Description |
经测试反应性 | Human, mouse |
免疫原 | c-Cbl fusion protein Ag23033 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | Cas-Br-M (murine) ecotropic retroviral transforming sequence |
别名 | C CBL, CBL, CBL2, Proto oncogene c Cbl, RING finger protein 55, RNF55 |
计算分子量 | 906 aa, 100 kDa |
观测分子量 | 100-120 kDa |
GenBank蛋白编号 | BC132733 |
基因名称 | CBL |
Gene ID (NCBI) | 867 |
RRID | AB_3084895 |
偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
最大激发/发射波长 | 755 nm / 780 nm |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P22681 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
c-Cbl (Casitas B-lineage lymphoma) is a critical regulator that controls immature or follicular helper T cell development, B cell development, immune tolerance, angiogenesis, or tumorigenesis (PMID: 33962939). c-Cbl also plays a pivotal role in regulating dendritic cell (DC) function via negatively modulating IL-12 production after Toll-like receptor (TLR) stimulation (PMID: 21799517).
实验方案
Product Specific Protocols | |
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WB protocol for CL Plus 750 c-Cbl antibody CL750-66576 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |