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验证数据展示

  • WB analysis using CL750-66576

    WB analysis using CL750-66576

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-66576 (c-Cbl antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 750-conjugated c-Cbl Monoclonal antibody

c-Cbl Monoclonal Antibody for WB
Cat No. CL750-66576

Print datasheet

CloneNo. 2F6D1

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB

Synonyms

C CBL, CBL, CBL2, Proto oncogene c Cbl, RING finger protein 55, RNF55

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 750
Size: 

-/ -

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经过测试的应用

Positive WB detected inRaji cells, mouse brain tissue, A431 cells
Planning an IHC experiment? We recommend our IHCeasy CBL Ready-To-Use IHC Kit. CBL primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66576-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-66576 targets c-Cbl in WB applications and shows reactivity with Human, mouse samples.

Tested Applications WB Application Description
Tested Reactivity Human, mouse
Immunogen c-Cbl fusion protein Ag23033 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Cas-Br-M (murine) ecotropic retroviral transforming sequence
Synonyms C CBL, CBL, CBL2, Proto oncogene c Cbl, RING finger protein 55, RNF55
Calculated Molecular Weight 906 aa, 100 kDa
Observed Molecular Weight 100-120 kDa
GenBank Accession NumberBC132733
Gene Symbol CBL
Gene ID (NCBI) 867
RRIDAB_3084895
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP22681
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

c-Cbl (Casitas B-lineage lymphoma) is a critical regulator that controls immature or follicular helper T cell development, B cell development, immune tolerance, angiogenesis, or tumorigenesis (PMID: 33962939). c-Cbl also plays a pivotal role in regulating dendritic cell (DC) function via negatively modulating IL-12 production after Toll-like receptor (TLR) stimulation (PMID: 21799517).

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 c-Cbl antibody CL750-66576Download protocol
Standard Protocols
Click here to view our Standard Protocols