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CoraLite® Plus 750-conjugated c-Cbl Monoclonal antibody
c-Cbl Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse
Applications
WB
Conjugate
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
2F6D1
验证数据展示
经过测试的应用
| Positive WB detected in | Raji cells, mouse brain tissue, A431 cells |
Planning an IHC experiment? We recommend our IHCeasy CBL Ready-To-Use IHC Kit. CBL primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66576-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-66576 targets c-Cbl in WB applications and shows reactivity with Human, mouse samples.
| Tested Applications | WB Application Description |
| Tested Reactivity | Human, mouse |
| Immunogen | c-Cbl fusion protein Ag23033 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | Cas-Br-M (murine) ecotropic retroviral transforming sequence |
| Synonyms | C CBL, CBL, CBL2, Proto oncogene c Cbl, RING finger protein 55, RNF55 |
| Calculated Molecular Weight | 906 aa, 100 kDa |
| Observed Molecular Weight | 100-120 kDa |
| GenBank Accession Number | BC132733 |
| Gene Symbol | CBL |
| Gene ID (NCBI) | 867 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P22681 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
c-Cbl (Casitas B-lineage lymphoma) is a critical regulator that controls immature or follicular helper T cell development, B cell development, immune tolerance, angiogenesis, or tumorigenesis (PMID: 33962939). c-Cbl also plays a pivotal role in regulating dendritic cell (DC) function via negatively modulating IL-12 production after Toll-like receptor (TLR) stimulation (PMID: 21799517).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 c-Cbl antibody CL750-66576 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |