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c-Met (Cytoplasmic) Recombinant antibody, PBS Only

c-Met (Cytoplasmic) Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G12

Cat No : 82904-1-PBS

Print datasheet

Synonyms

AUTS9, c Met, HGF receptor, HGF/SF receptor, HGFR, MET, Proto oncogene c Met, RCCP2, Scatter factor receptor, SF receptor, Tyrosine protein kinase Met

验证数据展示

  • WB analysis of HepG2 using 82904-1-RR (same clone as 82904-1-PBS)

    WB analysis of HepG2 using 82904-1-RR (same clone as 82904-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 82904-1-RR (c-Met antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82904-1-PBS in a different storage buffer formulation.

  • WB analysis using 82904-1-RR (same clone as 82904-1-PBS)

    WB analysis using 82904-1-RR (same clone as 82904-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82904-1-RR (c-Met antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82904-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 82904-1-RR (same clone as 82904-1-PBS)

    WB analysis of HEK-293 using 82904-1-RR (same clone as 82904-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 82904-1-RR (c-Met antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82904-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy MET Ready-To-Use IHC Kit. MET primary antibody included.

产品信息

82904-1-PBS targets c-Met (Cytoplasmic) in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen c-Met (Cytoplasmic) fusion protein Ag23140 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name met proto-oncogene (hepatocyte growth factor receptor)
Synonyms AUTS9, c Met, HGF receptor, HGF/SF receptor, HGFR, MET, Proto oncogene c Met, RCCP2, Scatter factor receptor, SF receptor, Tyrosine protein kinase Met
Calculated Molecular Weight 1390 aa, 155 kDa
Observed Molecular Weight140 kDa
GenBank Accession NumberBC130420
Gene Symbol MET
Gene ID (NCBI) 4233
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08581
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

c-Met (also named MET or HGFR) is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to the HGF ligand. c-Met regulates many physiological processes including proliferation, scattering, morphogenesis, and survival. The primary single-chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide-linked to form the mature receptor. Overexpression and/or mutation of c-Met has been reported in various human malignancies, including lung cancer, breast cancer, head and neck cancer, gastric cancer, colorectal cancer, bladder cancer, uterine cervix carcinoma, esophageal carcinoma, c-Met could serve as an important therapeutic target (PMID: 26036285). The c-met receptor is a 190-kD glycoprotein consisting of a 145-kD membrane-spanning beta chain and a 50-kD alpha chain (PMID: 7806559). In Western blot, this antibody produces bands of unknown identity at 55 and 100 kDa.