验证数据展示
经过测试的应用
| Positive WB detected in | NK-92 cells, NCI-H1299 cells, SCaBER cells, A431 cells, THP-1 cells, Mo7e cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
68640-1-Ig targets cGAS in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | mouse |
| 免疫原 | cGAS fusion protein Ag30074 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 6 open reading frame 150 |
| 别名 | 2'3'-cGAMP synthase, 2G2C5, C6orf150, cGAMP synthase, EC:2.7.7.86 |
| 计算分子量 | 496 aa, 54 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | BC113608 |
| 基因名称 | cGAS |
| Gene ID (NCBI) | 115004 |
| RRID | AB_3085328 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8N884 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
cGAS (Cyclic GMP-AMP synthase), also known as C6orf150 or h-cGAS, is a 522 aa protein. cGAS mediates innate immune responses against invading pathogens, or against self-dsDNA, which causes autoimmune disorders. The cGAS sensor not only recognizes cytosolic dsDNA but also synthesizes the second messenger cGAMP from ATP and GTP, which then binds to and activates STING. STING undergoes conformational changes and translocation from the endoplasmic reticulum to the Golgi apparatus to encounter TBK1 and IRF3, eventually triggering the production of type I IFNs.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for cGAS antibody 68640-1-Ig | Download protocol |
| IF protocol for cGAS antibody 68640-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
