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cGAS Monoclonal antibody, PBS Only

cGAS Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G2C5

Cat no : 68640-1-PBS

Print datasheet

Synonyms

h-cGAS, EC:2.7.7.86, cGAMP synthase, C6orf150, 2G2C5

验证数据展示

  • WB analysis using 68640-1-Ig (same clone as 68640-1-PBS)

    WB analysis using 68640-1-Ig (same clone as 68640-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68640-1-Ig (cGAS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68640-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68640-1-Ig (same clone as 68640-1-PBS)

    IF Staining of HeLa using 68640-1-Ig (same clone as 68640-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using cGAS antibody (68640-1-Ig, Clone: 2G2C5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68640-1-PBS in a different storage buffer formulation.

产品信息

68640-1-PBS targets cGAS in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen cGAS fusion protein Ag30074 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name chromosome 6 open reading frame 150
Synonyms h-cGAS, EC:2.7.7.86, cGAMP synthase, C6orf150, 2G2C5
Calculated Molecular Weight 496 aa, 54 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC113608
Gene Symbol cGAS
Gene ID (NCBI) 115004
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8N884
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

cGAS (Cyclic GMP-AMP synthase), also known as C6orf150 or h-cGAS, is a 522 aa protein. cGAS mediates innate immune responses against invading pathogens, or against self-dsDNA, which causes autoimmune disorders. The cGAS sensor not only recognizes cytosolic dsDNA but also synthesizes the second messenger cGAMP from ATP and GTP, which then binds to and activates STING. STING undergoes conformational changes and translocation from the endoplasmic reticulum to the Golgi apparatus to encounter TBK1 and IRF3, eventually triggering the production of type I IFNs.