验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, RAW 264.7 cells, C2C12 cells |
| Positive IP detected in | NIH/3T3 cells |
| Positive IF/ICC detected in | RAW 264.7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84045-1-RR targets cGAS in WB, IF/ICC, IP, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 经测试反应性 | mouse |
| 免疫原 | cGAS fusion protein Ag31677 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | RIKEN cDNA E330016A19 gene |
| 别名 | 240964E2, m cGAS, Mb21d1, mcGAS, m-cGAS |
| 计算分子量 | 58 kDa |
| 观测分子量 | 58 kDa |
| GenBank蛋白编号 | BC145651 |
| 基因名称 | cGAS |
| Gene ID (NCBI) | 214763 |
| RRID | AB_3671612 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q8C6L5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
cGAS (cyclic GMP-AMP synthase) is a cytosolic DNA sensor that serves to mount an immune response against the invasion of microbial pathogens such as viruses. cGAS normally resides as an inactive protein in the cell. Upon binding to DNA, cGAS undergoes a conformational change to an active state and produces the second messenger cyclic GMP-AMP (cGAMP) from ATP and GTP, which is subsequently detected by the cyclic-dinucleotide sensor STING, an ~40 kDa dimeric transmembrane protein at the endoplasmic reticulum (ER). cGAS not only is found in the cytosol but has a multifaceted cellular distribution that involves localization at the cell membrane and in the nucleus (PMID: 32424334). The calculated molecular weight of cGAS is 58 kDa. With post-translational modification, the MW of cGAS will be migrated to 70 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for cGAS antibody 84045-1-RR | Download protocol |
| IF protocol for cGAS antibody 84045-1-RR | Download protocol |
| IP protocol for cGAS antibody 84045-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |