验证数据展示
经过测试的应用
Positive IF/ICC detected in | RAW 264.7 cells |
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-84045 targets cGAS in IF/ICC applications and shows reactivity with mouse samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | mouse |
Immunogen | cGAS fusion protein Ag31677 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | RIKEN cDNA E330016A19 gene |
Synonyms | m-cGAS, mcGAS, Mb21d1, m cGAS, 240964E2 |
Calculated Molecular Weight | 58 kDa |
Observed Molecular Weight | 58 kDa |
GenBank Accession Number | BC145651 |
Gene Symbol | cGAS |
Gene ID (NCBI) | 214763 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q8C6L5 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
cGAS (cyclic GMP-AMP synthase) is a cytosolic DNA sensor that serves to mount an immune response against the invasion of microbial pathogens such as viruses. cGAS normally resides as an inactive protein in the cell. Upon binding to DNA, cGAS undergoes a conformational change to an active state and produces the second messenger cyclic GMP-AMP (cGAMP) from ATP and GTP, which is subsequently detected by the cyclic-dinucleotide sensor STING, an ~40 kDa dimeric transmembrane protein at the endoplasmic reticulum (ER). cGAS not only is found in the cytosol but has a multifaceted cellular distribution that involves localization at the cell membrane and in the nucleus (PMID: 32424334). The calculated molecular weight of cGAS is 58 kDa. With post-translational modification, the MW of cGAS will be migrated to 70 kDa.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 cGAS antibody CL488-84045 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |