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验证数据展示

  • WB analysis using 68225-1-Ig

    WB analysis using 68225-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68225-1-Ig (CAPS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of U-251 using 68225-1-Ig

    IF Staining of U-251 using 68225-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using calcyphosine antibody (68225-1-Ig, Clone: 2B7D1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

calcyphosine Monoclonal antibody

calcyphosine Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68225-1-Ig

产品说明书

CloneNo. 2B7D1

宿主/亚型

Mouse / IgG1

种属反应性

Human, pig, rabbit

应用

WB, IF/ICC, ELISA

别名

Calcyphosin, calcyphosine, CAPS, CAPS1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inSK-BR-3 cells, pig lung tissue, rabbit lung tissue, U-251 cells, PC-3 cells
Positive IF/ICC detected inU-251 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68225-1-Ig targets calcyphosine in WB, IF/ICC, ELISA applications and shows reactivity with Human, pig, rabbit samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, pig, rabbit
免疫原 calcyphosine fusion protein Ag10330 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 calcyphosine
别名 Calcyphosin, calcyphosine, CAPS, CAPS1
计算分子量 189 aa, 21 kDa
观测分子量21 kDa
GenBank蛋白编号BC080599
基因名称 calcyphosine
Gene ID (NCBI) 828
RRIDAB_2935313
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ13938
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

calcyphosine is a calcium-binding protein, which may play a role in the regulation of ion transport. Calcyphosine (CAPS) was initially identified in the canine thyroid but has also been detected in humans and other mammals. CAPS synthesis is increased by thyroid‐stimulating hormone and cAMP analogs that promote cell proliferation and maintain cell differentiation. Recently, CAPS was found to be involved in many kinds of tumor.

实验方案

Product Specific Protocols
WB protocol for calcyphosine antibody 68225-1-IgDownload protocol
IF protocol for calcyphosine antibody 68225-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols