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Species Reactivity
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验证数据展示

  • WB analysis using 68225-1-Ig

    WB analysis using 68225-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68225-1-Ig (CAPS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of U-251 using 68225-1-Ig

    IF Staining of U-251 using 68225-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using calcyphosine antibody (68225-1-Ig, Clone: 2B7D1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

calcyphosine Monoclonal antibody

calcyphosine Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68225-1-Ig
CloneNo. 2B7D1

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rabbit

Applications

WB, IF/ICC, ELISA

Synonyms

Calcyphosin, calcyphosine, CAPS, CAPS1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inSK-BR-3 cells, pig lung tissue, rabbit lung tissue, U-251 cells, PC-3 cells
Positive IF/ICC detected inU-251 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68225-1-Ig targets calcyphosine in WB, IF/ICC, ELISA applications and shows reactivity with Human, pig, rabbit samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, pig, rabbit
Immunogen calcyphosine fusion protein Ag10330 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name calcyphosine
Synonyms Calcyphosin, calcyphosine, CAPS, CAPS1
Calculated Molecular Weight 189 aa, 21 kDa
Observed Molecular Weight21 kDa
GenBank Accession NumberBC080599
Gene Symbol calcyphosine
Gene ID (NCBI) 828
RRIDAB_2935313
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13938
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

calcyphosine is a calcium-binding protein, which may play a role in the regulation of ion transport. Calcyphosine (CAPS) was initially identified in the canine thyroid but has also been detected in humans and other mammals. CAPS synthesis is increased by thyroid‐stimulating hormone and cAMP analogs that promote cell proliferation and maintain cell differentiation. Recently, CAPS was found to be involved in many kinds of tumor.

实验方案

Product Specific Protocols
WB protocol for calcyphosine antibody 68225-1-IgDownload protocol
IF protocol for calcyphosine antibody 68225-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols