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验证数据展示

  • IF Staining of HeLa using CL555-67686

    IF Staining of HeLa using CL555-67686

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®555 cyclin B1 antibody (CL555-67686, Clone: 1F3G11 ) at dilution of 1:200.

  • FC experiment of HeLa using CL555-67686

    FC experiment of HeLa using CL555-67686

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human cyclin B1 (CL555-67686, Clone:1F3G11) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated cyclin B1 Monoclonal antibody

cyclin B1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL555-67686

Print datasheet

CloneNo. 1F3G11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

CCNB1, G2/mitotic-specific cyclin-B1, G2/mitotic specific cyclin B1, Cyclin B, CCNB

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®555
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CCNB1 Ready-To-Use IHC Kit. CCNB1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67686-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-67686 targets cyclin B1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen cyclin B1 fusion protein Ag29444 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cyclin B1
Synonyms CCNB1, G2/mitotic-specific cyclin-B1, G2/mitotic specific cyclin B1, Cyclin B, CCNB
Calculated Molecular Weight 48 kDa
Observed Molecular Weight55 kDa
GenBank Accession NumberBC006510
Gene Symbol Cyclin B1
Gene ID (NCBI) 891
RRIDAB_2934686
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP14635
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.

实验方案

Product Specific Protocols
IF protocol for CL555 cyclin B1 antibody CL555-67686Download protocol
FC protocol for CL555 cyclin B1 antibody CL555-67686Download protocol
Standard Protocols
Click here to view our Standard Protocols