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  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated eIF4G2/DAP5 Monoclonal antibody

eIF4G2/DAP5 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

3C11A3

Cat no : CL555-67428

Print datasheet

Synonyms

EIF4G2, Death associated protein 5, DAP-5, DAP5, DAP 5

验证数据展示

  • IF Staining of HepG2 using CL555-67428

    IF Staining of HepG2 using CL555-67428

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®555 eIF4G2/DAP5 antibody (CL555-67428, Clone: 3C11A3 ) at dilution of 1:600.

  • FC experiment of HeLa using CL555-67428

    FC experiment of HeLa using CL555-67428

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555-conjugated eIF4G2/DAP5 Monoclonal antibody (CL555-67428, Clone:3C11A3)(red), or 0.4 ug CoraLite® Plus 555 Mouse IgG2a Isotype Control (C1.18.4) (CL555-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy EIF4G2 Ready-To-Use IHC Kit. EIF4G2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67428-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-67428 targets eIF4G2/DAP5 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen eIF4G2/DAP5 fusion protein Ag12080 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name eukaryotic translation initiation factor 4 gamma, 2
Synonyms EIF4G2, Death associated protein 5, DAP-5, DAP5, DAP 5
Calculated Molecular Weight 907 aa, 102 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC043149
Gene Symbol EIF4G2
Gene ID (NCBI) 1982
RRIDAB_2919702
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP78344
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL555 eIF4G2/DAP5 antibody CL555-67428Download protocol
Standard Protocols
Click here to view our Standard Protocols