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FLNC Polyclonal antibody

FLNC Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28492-1-AP

Print datasheet

Synonyms

FLN2, FilaminC, Filamin C, Filamin 2, ABPL

验证数据展示

  • WB analysis of SGC-7901 using 28492-1-AP

    WB analysis of SGC-7901 using 28492-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 28492-1-AP (filamin-c antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 28492-1-AP

    IHC staining of human colon using 28492-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon slide using 28492-1-AP (FLNC antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inSGC-7901 cells
Positive IHC detected inhuman colon
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

28492-1-AP targets FLNC in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen FLNC fusion protein Ag29328 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name filamin C, gamma (actin binding protein 280)
Synonyms FLN2, FilaminC, Filamin C, Filamin 2, ABPL
Calculated Molecular Weight 291 kDa
Observed Molecular Weight 280-300 kDa
GenBank Accession NumberNM_001458
Gene Symbol FLNC
Gene ID (NCBI) 2318
RRIDAB_2918171
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14315
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Filamin C (FLNC; also known as γ-FLN; ABP-L; FLN2), a muscle-specific filamin and a large actin-cross-linking protein. Human filamins are ~280 kDa homodimers, each monomer consisting of an N-terminal actin-binding domain followed by 24 immunoglobulin (Ig)-like domains (d1 to d24), the last of which mediates dimerization. FLNC is specifically expressed in cardiomyocytes and skeletal myocytes and is involved in the maintenance of structural integrity. High filamin C associated with better prognosis of prostate cancer, leukemia and breast cancer patients. ( PMID: 25577646, PMID: 30867563, PMID: 31131323)

实验方案

Product Specific Protocols
WB protocol for FLNC antibody 28492-1-APDownload protocol
IHC protocol for FLNC antibody 28492-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Mol Sci

A Regulator Role for the ATP-Binding Cassette Subfamily C Member 6 Transporter in HepG2 Cells: Effect on the Dynamics of Cell-Cell and Cell-Matrix Interactions

Authors - Ilenia Matera
View Article