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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 12681-1-AP (same clone as 12681-1-PBS)

    WB analysis using 12681-1-AP (same clone as 12681-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12681-1-AP (gephyrin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12681-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 12681-1-AP (same clone as 12681-1-PBS)

    IHC staining of rat brain using 12681-1-AP (same clone as 12681-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12681-1-AP (gephyrin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12681-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 12681-1-AP (same clone as 12681-1-PBS)

    IHC staining of rat brain using 12681-1-AP (same clone as 12681-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12681-1-AP (gephyrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12681-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 12681-1-AP (same clone as 12681-1-PBS)

    IP experiment of HEK-293 using 12681-1-AP (same clone as 12681-1-PBS)

    IP result of anti-gephyrin (IP:12681-1-AP, 4ug; Detection:12681-1-AP 1:700) with HEK-293 cells lysate 3200ug. This data was developed using the same antibody clone with 12681-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 12681-1-AP (same clone as 12681-1-PBS)

    IF Staining of HEK-293 using 12681-1-AP (same clone as 12681-1-PBS)

    Immunofluorescent analysis of HEK-293 cells using 12681-1-AP (gephyrin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12681-1-PBS in a different storage buffer formulation.

gephyrin Polyclonal antibody, PBS Only

gephyrin Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12681-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

12681-1-PBS targets gephyrin in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 gephyrin fusion protein Ag3370 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 gephyrin
别名
计算分子量 769 aa, 83 kDa
观测分子量 93 kDa
GenBank蛋白编号BC030016
基因名称 GPHN
Gene ID (NCBI) 10243
RRIDAB_2247770
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQX3
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Gephyrin (GPHN) is an organizational protein that clusters and localizes the inhibitory glycine receptor (GlyR) and GABAA receptors to the microtubular matrix of the neuronal postsynaptic membrane. Mice deficient in gephyrin develop a hereditary molybdenum cofactor deficiency and a neurological phenotype that mimics startle disease (hyperekplexia). In non-neuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Two isoforms produced by alternative splicing have been described. The observed MW of Gephyrin is 93 kDa, larger than the predicated of 83 kDa, which may be due to the modifications on various phosphorylation sites.