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CoraLite® Plus 488-conjugated hIST1 Monoclonal antibody

hIST1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E12A7

Cat no : CL488-66989

Print datasheet

Synonyms

hIST1, IST1, IST1 homolog, KIAA0174, OLC1

验证数据展示

  • IF Staining of HeLa using CL488-66989

    IF Staining of HeLa using CL488-66989

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 hIST1 antibody (CL488-66989, Clone: 1E12A7 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy IST1 Ready-To-Use IHC Kit. IST1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66989-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66989 targets hIST1 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen hIST1 fusion protein Ag28364 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name KIAA0174
Synonyms hIST1, IST1, IST1 homolog, KIAA0174, OLC1
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC000430
Gene Symbol hIST1
Gene ID (NCBI) 9798
RRIDAB_2919401
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP53990
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human increased sodium tolerance-1 (hIST1), encoded by KIAA0174 gene, is a newly reported endosomal sorting complexes required for transport (ESCRT)-III family member.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 hIST1 antibody CL488-66989Download protocol
Standard Protocols
Click here to view our Standard Protocols