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  • Featured Product
  • KD/KO Validated

hnRNP-E1 Polyclonal antibody, PBS Only

hnRNP-E1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14523-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 14523-1-AP (same clone as 14523-1-PBS)

    WB analysis using 14523-1-AP (same clone as 14523-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 14523-1-AP (same clone as 14523-1-PBS)

    IHC staining of human lung cancer using 14523-1-AP (same clone as 14523-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PCBP1 antibody included
  • IHC staining of human lung cancer using 14523-1-AP (same clone as 14523-1-PBS)

    IHC staining of human lung cancer using 14523-1-AP (same clone as 14523-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PCBP1 antibody included
  • IF Staining of HepG2 using 14523-1-AP (same clone as 14523-1-PBS)

    IF Staining of HepG2 using 14523-1-AP (same clone as 14523-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using hnRNP-E1 antibody (14523-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 14523-1-AP (same clone as 14523-1-PBS)

    IF Staining of HeLa using 14523-1-AP (same clone as 14523-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using hnRNP-E1 antibody (14523-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PCBP1 Ready-To-Use IHC Kit. PCBP1 primary antibody included.

产品信息

14523-1-PBS targets hnRNP-E1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen hnRNP-E1 fusion protein Ag6007 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name poly(rC) binding protein 1
Synonyms
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC039742
Gene Symbol PCBP1
Gene ID (NCBI) 5093
RRIDAB_2160619
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15365
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

hnRNP-E1 (heterogeneous nuclear ribonucleoprotein E1) is also named as PCBP1 and Alpha-CP1. Poly(rC)-binding protein 1 (PCBP1 also called hnRNP E1) belongs to a hnRNP family that is composed of hnRNP K/J and the alpha-complex proteins (PCBP1-4α or CP1-4). Proteins in this family contain three hnRNP K homology (KH) domains for RNA-binding. The human PCBP1 gene encodes the hnRNP E1 protein, and was initially defined as clone sub2.3, with poly(C)-binding activity and observed similarity to hnRNP E2 (PMID: 8152927). hnRNP-E1 is an RNA binding protein that regulates diverse processes including translation and splicing (PMID:29396052). hnRNP E1 regulates splicing of EMT-associated genes including CD44 and PNUTS (PMID: 28825698). This antibody recognizes hnRNP E1/E2.