验证数据展示
经过测试的应用
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse colon tissue, mouse spleen tissue |
| Positive IF-Fro detected in | mouse spleen tissue, mouse liver tissue |
| Positive IF/ICC detected in | NIH/3T3 cells, HeLa cells |
| Positive FC (Intra) detected in | NIH/3T3 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 594 and CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:1500-1:6000 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28074-1-AP targets Ki-67 in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA, Cell treatment applications and shows reactivity with mouse samples.
| Tested Applications | IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA Application Description |
| Cited Applications | WB, IHC, IF, Cell treatment |
| Tested Reactivity | mouse |
| Cited Reactivity | mouse, rat |
| Immunogen | Ki-67 fusion protein Ag27894 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | antigen identified by monoclonal antibody Ki 67 |
| Synonyms | Mki67, ki67, Ki 67, Proliferation related Ki 67 antigen |
| Calculated Molecular Weight | 351 kDa |
| GenBank Accession Number | NM_001081117 |
| Gene Symbol | ki67 |
| Gene ID (NCBI) | 17345 |
| RRID | AB_2918145 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | E9PVX6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Ki-67 antibody 28074-1-AP | Download protocol |
| IF protocol for Ki-67 antibody 28074-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Drug Resist Updat Lansoprazole (LPZ) reverses multidrug resistance (MDR) in cancer through impeding ATP-binding cassette (ABC) transporter-mediated chemotherapeutic drug efflux and lysosomal sequestration | ||
Cell Mol Immunol Targeting PHGDH reverses the immunosuppressive phenotype of tumor-associated macrophages through α-ketoglutarate and mTORC1 signaling | ||
Biosens Bioelectron 3D co-culture of macrophages and fibroblasts in a sessile drop array for unveiling the role of macrophages in skin wound-healing | ||
Adv Healthc Mater A Multifunctional Nanodrug Increases the Therapeutic Sensitivity of Lenvatinib to Hepatocellular Carcinoma by Inhibiting the Stemness of Hepatic Cancer Stem Cells | ||
Cancer Lett Icaritin with autophagy/mitophagy inhibitors synergistically enhances anticancer efficacy and apoptotic effects through PINK1/Parkin-mediated mitophagy in hepatocellular carcinoma | ||
ACS Appl Mater Interfaces Doxorubicin-Loaded Microalgal Delivery System for Combined Chemotherapy and Enhanced Photodynamic Therapy of Osteosarcoma |
