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CoraLite® Plus 647-conjugated Ki-67 Polyclonal antibody

Ki-67 Polyclonal Antibody for IF/ICC, IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF/ICC, IF-P, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-28074

Print datasheet

Synonyms

Mki67, ki67, Ki 67, Proliferation related Ki 67 antigen

验证数据展示

  • IF Staining of mouse colon using CL647-28074

    IF Staining of mouse colon using CL647-28074

    Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CoraLite® Plus 647 ki67 antibody (CL647-28074) at dilution of 1:200.

  • IF Staining of mouse colon using CL647-28074

    IF Staining of mouse colon using CL647-28074

    Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CoraLite® Plus 647 ki67 antibody (CL647-28074) at dilution of 1:200.

  • IF Staining of HeLa using CL647-28074

    IF Staining of HeLa using CL647-28074

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 647 ki67 antibody (CL647-28074) at dilution of 1:200.

  • FC experiment of NIH/3T3 using CL647-28074

    FC experiment of NIH/3T3 using CL647-28074

    1x10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated Ki-67 Polyclonal antibody (CL647-28074)(red), or 0.4 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF-P detected inmouse colon tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inNIH/3T3 cells, Jurkat cells
For other applications, we recommend the unconjugated version of this antibody, 28074-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-28074 targets Ki-67 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with mouse samples.

Tested Applications IF/ICC, IF-P, FC (Intra) Application Description
Tested Reactivity mouse
Immunogen Ki-67 fusion protein Ag27894 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name antigen identified by monoclonal antibody Ki 67
Synonyms Mki67, ki67, Ki 67, Proliferation related Ki 67 antigen
Calculated Molecular Weight 351 kDa
GenBank Accession NumberNM_001081117
Gene Symbol ki67
Gene ID (NCBI) 17345
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDE9PVX6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 Ki-67 antibody CL647-28074Download protocol
Standard Protocols
Click here to view our Standard Protocols