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验证数据展示

  • IF Staining of mouse colon using CL647-28074

    IF Staining of mouse colon using CL647-28074

    Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CoraLite® Plus 647 ki67 antibody (CL647-28074) at dilution of 1:200.

  • IF Staining of mouse colon using CL647-28074

    IF Staining of mouse colon using CL647-28074

    Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CoraLite® Plus 647 ki67 antibody (CL647-28074) at dilution of 1:200.

  • IF Staining of HeLa using CL647-28074

    IF Staining of HeLa using CL647-28074

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 647 ki67 antibody (CL647-28074) at dilution of 1:200.

  • FC experiment of NIH/3T3 using CL647-28074

    FC experiment of NIH/3T3 using CL647-28074

    1x10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated Ki-67 Polyclonal antibody (CL647-28074)(red), or 0.4 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 647-conjugated Ki-67 Polyclonal antibody

Ki-67 Polyclonal Antibody for IF/ICC, IF-P, FC (Intra)
Cat No. CL647-28074

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

IF/ICC, IF-P, FC (Intra)

别名

Mki67, ki67, Ki 67, Proliferation related Ki 67 antigen

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse colon tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inNIH/3T3 cells, Jurkat cells
For other applications, we recommend the unconjugated version of this antibody, 28074-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-28074 targets Ki-67 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with mouse samples.

经测试应用 IF/ICC, IF-P, FC (Intra) Application Description
经测试反应性 mouse
免疫原 Ki-67 fusion protein Ag27894 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 antigen identified by monoclonal antibody Ki 67
别名 Mki67, ki67, Ki 67, Proliferation related Ki 67 antigen
计算分子量 351 kDa
GenBank蛋白编号NM_001081117
基因名称 ki67
Gene ID (NCBI) 17345
RRIDAB_3673572
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDE9PVX6
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 Ki-67 antibody CL647-28074Download protocol
Standard Protocols
Click here to view our Standard Protocols