5-methylcytosine Monoclonal antibody
5-methylcytosine Monoclonal Antibody for IHC, Dot Blot, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
5mc, chemical compound, m5c and More (2)
Applications
IHC, Dot Blot, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
2C9G9
验证数据展示
经过测试的应用
Positive IHC detected in | mouse testis tissue, rat brain tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive ELISA detected in | compound |
Positive Dot Blot detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
Enzyme-linked Immunosorbent Assay (ELISA) | ELISA : 1:10-1:100 |
DOT BLOT | DOT BLOT : 1:2500-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 1 publications below |
产品信息
68301-1-Ig targets 5-methylcytosine in IHC, IF, Dot Blot, ELISA applications and shows reactivity with 5mc, chemical compound, m5c samples.
Tested Applications | IHC, Dot Blot, ELISA Application Description |
Cited Applications | IF |
Tested Reactivity | 5mc, chemical compound, m5c |
Cited Reactivity | human, mouse |
Immunogen | Compound 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | 5-methylcytosine |
Synonyms | m5C, 5mC |
Gene Symbol | |
Gene ID (NCBI) | |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protocol for Dot Blot:
https://www.ptglab.com/protocol/68301-1-IgDotBlot.pdf
实验方案
Product Specific Protocols | |
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IHC protocol for 5-methylcytosine antibody 68301-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cancer Lett NSUN4 mediated RNA 5-methylcytosine promotes the malignant progression of glioma through improving the CDC42 mRNA stabilization | ||
Eur J Pharmacol 5- methylcytidine effectively improves spermatogenesis recovery in busulfan-induced oligoasthenospermia mice | ||
Cancer Biol Ther Function of m5C RNA methyltransferase NOP2 in high-grade serous ovarian cancer |