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CoraLite® Plus 488-conjugated mDia1 Polyclonal antibody
mDia1 Polyclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, monkey
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy DIAPH1 Ready-To-Use IHC Kit. DIAPH1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 20624-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-20624 targets mDia1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, monkey samples.
| Tested Applications | IF/ICC, FC (Intra) Application Description |
| Tested Reactivity | human, mouse, rat, monkey |
| Immunogen | mDia1 fusion protein Ag14523 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | diaphanous homolog 1 (Drosophila) |
| Synonyms | DIAPH1, Diaphanous-related formin-1, Diaphanous related formin 1, DIAPH 1, DIAP1 |
| Calculated Molecular Weight | 1272 aa, 141 kDa |
| Observed Molecular Weight | 140-150 kDa, 70 kDa |
| GenBank Accession Number | BC007411 |
| Gene Symbol | mDia1 |
| Gene ID (NCBI) | 1729 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60610 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
mDia1, also known as DIAPH1 or Diap1, is a mammalian diaphanous-related formin which is implicated in multiple physical and pathological events including cytoskeletal dynamics, autosomal hearing loss, and myelodysplasia. Depending upon the cell type and position in the cell cycle, mDia1 has been shown to localize to the cell cortex, trafficking endosomes, cleavage furrow, mid-bodies, and centrosomes, the cytoplasmic microtubule-organizing center crucial for cell division. Mutation of mDia1 has been linked to microcephaly. This antibody recognizes the endogenous mDia1 mainly around 140-150 kDa, while sometimes an additional 70 kDa can also be observed which is proposed to be a fragment of 140-150 kDa molecules (26011179).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 mDia1 antibody CL488-20624 | Download protocol |
| FC protocol for CL Plus 488 mDia1 antibody CL488-20624 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
