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验证数据展示

  • IHC staining of mouse heart using 18984-1-AP (same clone as 18984-1-PBS)

    IHC staining of mouse heart using 18984-1-AP (same clone as 18984-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18984-1-AP (nNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18984-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 18984-1-AP (same clone as 18984-1-PBS)

    IHC staining of mouse heart using 18984-1-AP (same clone as 18984-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18984-1-AP (nNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18984-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 18984-1-AP (same clone as 18984-1-PBS)

    IF Staining of mouse brain using 18984-1-AP (same clone as 18984-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using nNOS antibody (18984-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18984-1-PBS in a different storage buffer formulation.

nNOS Polyclonal antibody, PBS Only

nNOS Polyclonal Antibody for IHC, IF-P, Indirect ELISA
Cat No. 18984-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF-P, Indirect ELISA

别名

NOS1, N-NOS, NC-NOS, NC NOS, N NOS

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

18984-1-PBS targets nNOS in IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nitric oxide synthase 1 (neuronal)
别名 NOS1, N-NOS, NC-NOS, NC NOS, N NOS
计算分子量 161 kDa
GenBank蛋白编号NM_000620
基因名称 nNOS
Gene ID (NCBI) 4842
RRIDAB_10598169
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP29475
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

nNOS belongs to the NOS family. nNOS produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. NO is synthesized from L-arginine by nitric oxide synthases. nNOS is a nitric oxide synthase which is highly expressed in skeletal muscle. Genetic variations in nNOS gene are associated with susceptibility to infantile hypertrophic pyloric stenosis type 1 (IHPS1). The antibody can recognize isoform 1,2,4 of nNOS.