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CoraLite® Plus 488-conjugated nNOS Polyclonal antibody

nNOS Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-29231

Print datasheet

Synonyms

NOS1, N-NOS, NC-NOS, NC NOS, N NOS

验证数据展示

  • FC experiment of HeLa using CL488-29231

    FC experiment of HeLa using CL488-29231

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human nNOS (CL488-29231) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 29231-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-29231 targets nNOS in FC (Intra) applications and shows reactivity with mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity mouse, rat
Immunogen nNOS fusion protein Ag28910 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nitric oxide synthase 1 (neuronal)
Synonyms NOS1, N-NOS, NC-NOS, NC NOS, N NOS
Calculated Molecular Weight 161 kDa
Observed Molecular Weight160 kDa
GenBank Accession NumberNM_000620
Gene Symbol nNOS
Gene ID (NCBI) 4842
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29475
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

nNOS belongs to the NOS family. nNOS produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. NO is synthesized from L-arginine by nitric oxide synthases. nNOS is a nitric oxide synthase which is highly expressed in skeletal muscle. Genetic variations in nNOS gene are associated with susceptibility to infantile hypertrophic pyloric stenosis type 1 (IHPS1).The antibody can recognize isoform 1,2,4 of nNOS.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 nNOS antibody CL488-29231Download protocol
Standard Protocols
Click here to view our Standard Protocols