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验证数据展示

  • FC experiment of HeLa using CL488-29231

    FC experiment of HeLa using CL488-29231

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human nNOS (CL488-29231) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated nNOS Polyclonal antibody

nNOS Polyclonal Antibody for FC (Intra)
Cat No. CL488-29231

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

FC (Intra)

别名

NOS1, N-NOS, NC-NOS, NC NOS, N NOS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 29231-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-29231 targets nNOS in FC (Intra) applications and shows reactivity with mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 mouse, rat
免疫原 nNOS fusion protein Ag28910 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nitric oxide synthase 1 (neuronal)
别名 NOS1, N-NOS, NC-NOS, NC NOS, N NOS
计算分子量 161 kDa
观测分子量160 kDa
GenBank蛋白编号NM_000620
基因名称 nNOS
Gene ID (NCBI) 4842
RRIDAB_3672837
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP29475
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

nNOS belongs to the NOS family. nNOS produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. NO is synthesized from L-arginine by nitric oxide synthases. nNOS is a nitric oxide synthase which is highly expressed in skeletal muscle. Genetic variations in nNOS gene are associated with susceptibility to infantile hypertrophic pyloric stenosis type 1 (IHPS1).The antibody can recognize isoform 1,2,4 of nNOS.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 nNOS antibody CL488-29231Download protocol
Standard Protocols
Click here to view our Standard Protocols