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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated p115, USO1 Monoclonal antibody

p115, USO1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B7D8

Cat no : CL488-68100

Print datasheet

Synonyms

P115, Protein USO1 homolog, TAP, USO1, VDP, Vesicle docking protein

验证数据展示

  • IF Staining of HeLa using CL488-68100

    IF Staining of HeLa using CL488-68100

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 p115, USO1 antibody (CL488-68100, Clone: 3B7D8 ) at dilution of 1:100.

经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy USO1 Ready-To-Use IHC Kit. USO1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68100-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68100 targets p115, USO1 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen p115, USO1 fusion protein Ag5543 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name USO1 homolog, vesicle docking protein (yeast)
Synonyms P115, Protein USO1 homolog, TAP, USO1, VDP, Vesicle docking protein
Calculated Molecular Weight 962 aa, 108 kDa
Observed Molecular Weight108 kDa
GenBank Accession NumberBC032654
Gene Symbol USO1
Gene ID (NCBI) 8615
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60763
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

p115, also known as USO1, TAP (transcytosis-associated protein) or VDP (vesicle docking protein) is a general vesicular transport factor and plays an important role at different steps of vesicular transport. It is a 962-residue peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase (PMID: 9478999). p115 forms stable homodimers (PMID: 19247479). Rab1 recruits p115 to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where p115 interacts directly with a select set of SNARE proteins (PMID: 10903204). p115 is required for intra-Golgi transport, and also functions in endoplasmic reticulum to Golgi trafficking, Golgi biogenesis and exocytotic transport (PMID: 19247479).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 p115, USO1 antibody CL488-68100Download protocol
Standard Protocols
Click here to view our Standard Protocols