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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated p120 Catenin Monoclonal antibody

p120 Catenin Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2F7H8

Cat no : CL594-66208

Print datasheet

Synonyms

CAS, Catenin delta 1, CTNND, CTNND1, KIAA0384, p120, p120 catenin, p120(cas), p120(ctn), P120CAS, P120CTN

验证数据展示

  • IF Staining of human breast cancer using CL594-66208

    IF Staining of human breast cancer using CL594-66208

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using the CoraLite®594-conjugated version of this antibody, CL594-66208 (p120 Catenin antibody), at dilution of 1:100.

  • IF Staining of human breast cancer using CL594-66208

    IF Staining of human breast cancer using CL594-66208

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using the CoraLite®594-conjugated version of this antibody, CL594-66208 (p120 Catenin antibody), at dilution of 1:100.

  • IF Staining of MCF-7 using CL594-66208

    IF Staining of MCF-7 using CL594-66208

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using CL594-66208 (p120 Catenin antibody) at dilution of 1:100.

经过测试的应用

Positive IF-P detected inhuman breast cancer tissue
Positive IF/ICC detected inMCF-7 cells, human breast cancer tissue
Planning an IHC experiment? We recommend our IHCeasy p120 Catenin Ready-To-Use IHC Kit. p120 Catenin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66208-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66208 targets p120 Catenin in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IF-P Application Description
Tested Reactivity human, mouse, rat
Immunogen p120 Catenin fusion protein Ag2824 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name catenin (cadherin-associated protein), delta 1
Synonyms CAS, Catenin delta 1, CTNND, CTNND1, KIAA0384, p120, p120 catenin, p120(cas), p120(ctn), P120CAS, P120CTN
Calculated Molecular Weight 948 aa, 105 kDa
Observed Molecular Weight 90-120 kDa
GenBank Accession NumberBC010501
Gene Symbol p120 Catenin
Gene ID (NCBI) 1500
RRIDAB_2883536
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60716
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Catenins were discovered as proteins that are linked to the cytoplasmic domain of transmembrane cadherins (PMID: 9653641). p120 catenin, also called p120 ctn or catenin delta-1, regulates cell-cell adhesion through its interaction with the cytoplasmic tail of classical and type II cadherins. p120 catenin is a tyrosine kinase substrate implicated in cell transformation by SRC, as well as in ligand-induced receptor signaling through the EGF receptor, the PDGF receptor, and the CSF1 receptor. Different expression patterns of p120 catenin in lobular and ductal carcinomas of breast have been reported: membrane stain for ductal carcinoma and cytoplasmic stain for lobular carcinoma (PMID: 24966968). Different isoforms of p120 catenin are variably expressed in different tissues as a result of alternative splicing and the use of multiple translation initiation codons (PMID: 19150613).

实验方案

Product Specific Protocols
IF protocol for CL594 p120 Catenin antibody CL594-66208Download protocol
Standard Protocols
Click here to view our Standard Protocols