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p130Cas / BCAR1 Monoclonal antibody, PBS Only

p130Cas / BCAR1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D11C10

Cat No : 67215-1-PBS

Print datasheet

Synonyms

BCAR1, CAS, CAS1, CASS1, CRK associated substrate, CRKAS, P130Cas

验证数据展示

  • WB analysis using 67215-1-Ig (same clone as 67215-1-PBS)

    WB analysis using 67215-1-Ig (same clone as 67215-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

    IHC staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

    IHC staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

    IF Staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using p130Cas / BCAR1 antibody (67215-1-Ig, Clone: 2D11C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

    IF Staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using BCAR1 antibody (67215-1-Ig, Clone: 2D11C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

产品信息

67215-1-PBS targets p130Cas / BCAR1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF, IHC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen p130Cas / BCAR1 fusion protein Ag10604 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name breast cancer anti-estrogen resistance 1
Synonyms BCAR1, CAS, CAS1, CASS1, CRK associated substrate, CRKAS, P130Cas
Calculated Molecular Weight 870 aa, 93 kDa
Observed Molecular Weight 116 kDa
GenBank Accession NumberBC062556
Gene Symbol BCAR1
Gene ID (NCBI) 9564
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP56945
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.