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p130Cas / BCAR1 Monoclonal antibody

p130Cas / BCAR1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF-P, FC (Intra), ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

2D11C10

Cat no : 67215-1-Ig

Print datasheet

Synonyms

BCAR1, 2D11C10, CASS1, CAS1, CAS

验证数据展示

  • WB analysis using 67215-1-Ig

    WB analysis using 67215-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67215-1-Ig

    IHC staining of human breast cancer using 67215-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 67215-1-Ig

    IHC staining of human breast cancer using 67215-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human breast cancer using 67215-1-Ig

    IF Staining of human breast cancer using 67215-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using p130Cas / BCAR1 antibody (67215-1-Ig, Clone: 2D11C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human breast cancer using 67215-1-Ig

    IF Staining of human breast cancer using 67215-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using BCAR1 antibody (67215-1-Ig, Clone: 2D11C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of A431 using 67215-1-Ig

    FC experiment of A431 using 67215-1-Ig

    1x10^6 A431 cells were intracellularly stained with 0.25 ug p130Cas / BCAR1 Monoclonal antibody (67215-1-Ig, Clone:2D11C10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHEK-293 cells, A431 cells, HeLa cells, MCF-7 cells, T-47D cells, MDA-MB-453s cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman breast cancer tissue
Positive FC (Intra) detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67215-1-Ig targets p130Cas / BCAR1 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF-P, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen p130Cas / BCAR1 fusion protein Ag10604 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name breast cancer anti-estrogen resistance 1
Synonyms BCAR1, 2D11C10, CASS1, CAS1, CAS
Calculated Molecular Weight 870 aa, 93 kDa
Observed Molecular Weight 116 kDa
GenBank Accession NumberBC062556
Gene Symbol BCAR1
Gene ID (NCBI) 9564
RRIDAB_2882507
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP56945
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for p130Cas / BCAR1 antibody 67215-1-IgDownload protocol
IHC protocol for p130Cas / BCAR1 antibody 67215-1-IgDownload protocol
IF protocol for p130Cas / BCAR1 antibody 67215-1-IgDownload protocol
FC protocol for p130Cas / BCAR1 antibody 67215-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Genet

LYPD3, a New Biomarker and Therapeutic Target for Acute Myelogenous Leukemia.

Authors - Tingting Hu
View Article
humanIF

Comput Math Methods Med

Protumor Effects of Histone H3-H4 Chaperone Antisilencing Feature 1B Gene on Lung Adenocarcinoma: In Silico and In Vitro Analyses.

Authors - Liyang Wu
View Article