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验证数据展示

  • FC experiment of A431 using CL647-67215

    FC experiment of A431 using CL647-67215

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human p130Cas / BCAR1 (CL647-67215, Clone:2D11C10) (red), or 0.4 ug CoraLite® Plus 647 Mouse IgG2a Isotype Control (C1.18.4) (CL647-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 647-conjugated p130Cas / BCAR1 Monoclonal antibody

p130Cas / BCAR1 Monoclonal Antibody for FC (Intra)
Cat No. CL647-67215

产品说明书

CloneNo. 2D11C10

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

FC (Intra)

别名

BCAR1, CAS, CAS1, CASS1, CRK associated substrate, CRKAS, P130Cas

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inA431 cells
Positive FC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 67215-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-67215 targets p130Cas / BCAR1 in FC (Intra) applications and shows reactivity with Human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human
免疫原 p130Cas / BCAR1 fusion protein Ag10604 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 breast cancer anti-estrogen resistance 1
别名 BCAR1, CAS, CAS1, CASS1, CRK associated substrate, CRKAS, P130Cas
计算分子量 870 aa, 93 kDa
观测分子量 116 kDa
GenBank蛋白编号BC062556
基因名称 BCAR1
Gene ID (NCBI) 9564
RRIDAB_2935074
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP56945
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 p130Cas / BCAR1 antibody CL647-67215Download protocol
Standard Protocols
Click here to view our Standard Protocols