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p38 MAPK Recombinant antibody

p38 MAPK Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

230263D11

Cat no : 80821-2-RR

Print datasheet

Synonyms

MAPK14, CSPB1, CSBP2, CSBP1, CSBP

验证数据展示

  • WB analysis using 80821-2-RR

    WB analysis using 80821-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80821-2-RR (p38 MAPK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 80821-2-RR

    IP experiment of HEK-293 using 80821-2-RR

    IP result of anti-p38 MAPK (IP:80821-2-RR, 4ug; Detection:80821-2-RR 1:2000) with HEK-293 cells lysate 1575 ug.

  • IF Staining of HeLa using 80821-2-RR

    IF Staining of HeLa using 80821-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using p38 MAPK antibody (80821-2-RR, Clone: 230263D11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HeLa using 80821-2-RR

    FC experiment of HeLa using 80821-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human p38 MAPK (80821-2-RR, Clone:230263D11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • FC experiment of A431 using 80821-2-RR

    FC experiment of A431 using 80821-2-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human p38 MAPK (80821-2-RR, Clone:230263D11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 80821-2-RR

    Affinity and Kinetic Characterization of 80821-2-RR

    Biolayer interferometry (BLl) kinetic assays of 80821-2-RR against Human p38 MAPK were performed. The affinity constant is 0.435 nM.

经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, HEK-293 cells, K-562 cells, NIH/3T3 cells, RAW 264.7 cells, PC-12 cells
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells, A431 cells
Planning an IHC experiment? We recommend our IHCeasy p38 MAPK Ready-To-Use IHC Kit. p38 MAPK primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80821-2-RR targets p38 MAPK in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, FC (Intra), IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen p38 MAPK fusion protein Ag5115 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mitogen-activated protein kinase 14
Synonyms MAPK14, CSPB1, CSBP2, CSBP1, CSBP
Calculated Molecular Weight 360 aa, 41 kDa
Observed Molecular Weight38-42 kDa
GenBank Accession NumberBC031574
Gene Symbol p38 MAPK
Gene ID (NCBI) 1432
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16539
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAPK14(mitogen-activated protein kinase 14) is also named as SAPK2A, p38MAPK, CSBP1, RK, p38, EXIP, Mxi2, CSBP2, PRKM14, PRKM15, CSPB1, p38ALPHA and belongs to the MAP kinase subfamily. MAPK14-signaling is a central pathway for the integration of instructive signals in dendritic cells for T(H)17 differentiation and inflammation(PMID:22231518). It plays an important role in the regulation of hematopoietic stem cell self-renewal in vitro and inhibition of MAPK14 activation with a small molecule inhibitor may represent a novel approach to promote ex vivo expansion of hematopoietic stem cell(PMID:21198398). This protein has 4 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for p38 MAPK antibody 80821-2-RRDownload protocol
IF protocol for p38 MAPK antibody 80821-2-RRDownload protocol
IP protocol for p38 MAPK antibody 80821-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols