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p38 MAPK Recombinant antibody, PBS Only

p38 MAPK Recombinant Antibody for WB, IP, IF, FC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IP, IF, FC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230263D11

Cat No : 80821-2-PBS

Print datasheet

Synonyms

CSAID binding protein, CSBP, CSBP1, CSBP2, CSPB1, EXIP, MAP kinase 14, MAP kinase MXI2, MAP kinase p38 alpha, MAPK 14, MAPK14, MAX interacting protein 2, Mxi2, p38, p38ALPHA, PRKM14, PRKM15, RK, SAPK2A

验证数据展示

  • WB analysis using 80821-2-RR (same clone as 80821-2-PBS)

    WB analysis using 80821-2-RR (same clone as 80821-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80821-2-RR (p38 MAPK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80821-2-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 80821-2-RR (same clone as 80821-2-PBS)

    IP experiment of HEK-293 using 80821-2-RR (same clone as 80821-2-PBS)

    IP result of anti-p38 MAPK (IP:80821-2-RR, 4ug; Detection:80821-2-RR 1:2000) with HEK-293 cells lysate 1575 ug. This data was developed using the same antibody clone with 80821-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 80821-2-RR (same clone as 80821-2-PBS)

    IF Staining of HepG2 using 80821-2-RR (same clone as 80821-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using p38 MAPK antibody (80821-2-RR, Clone: 230263D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80821-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 80821-2-RR (same clone as 80821-2-PBS)

    FC experiment of HeLa using 80821-2-RR (same clone as 80821-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human p38 MAPK (80821-2-RR, Clone:230263D11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 80821-2-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 80821-2-RR (same clone as 80821-2-PBS)

    FC experiment of A431 using 80821-2-RR (same clone as 80821-2-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human p38 MAPK (80821-2-RR, Clone:230263D11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 80821-2-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy p38 MAPK Ready-To-Use IHC Kit. p38 MAPK primary antibody included.

产品信息

80821-2-PBS targets p38 MAPK in WB, IP, IF, FC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IP, IF, FC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen p38 MAPK fusion protein Ag5115 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mitogen-activated protein kinase 14
Synonyms CSAID binding protein, CSBP, CSBP1, CSBP2, CSPB1, EXIP, MAP kinase 14, MAP kinase MXI2, MAP kinase p38 alpha, MAPK 14, MAPK14, MAX interacting protein 2, Mxi2, p38, p38ALPHA, PRKM14, PRKM15, RK, SAPK2A
Calculated Molecular Weight 360 aa, 41 kDa
Observed Molecular Weight38-42 kDa
GenBank Accession NumberBC031574
Gene Symbol MAPK14
Gene ID (NCBI) 1432
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16539
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

MAPK14(mitogen-activated protein kinase 14) is also named as SAPK2A, p38MAPK, CSBP1, RK, p38, EXIP, Mxi2, CSBP2, PRKM14, PRKM15, CSPB1, p38ALPHA and belongs to the MAP kinase subfamily. MAPK14-signaling is a central pathway for the integration of instructive signals in dendritic cells for T(H)17 differentiation and inflammation(PMID:22231518). It plays an important role in the regulation of hematopoietic stem cell self-renewal in vitro and inhibition of MAPK14 activation with a small molecule inhibitor may represent a novel approach to promote ex vivo expansion of hematopoietic stem cell(PMID:21198398). This protein has 4 isoforms produced by alternative splicing.