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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 80821-2-RR (same clone as 80821-2-PBS)

    WB analysis using 80821-2-RR (same clone as 80821-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80821-2-RR (p38 MAPK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80821-2-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 80821-2-RR (same clone as 80821-2-PBS)

    IP experiment of HEK-293 using 80821-2-RR (same clone as 80821-2-PBS)

    IP result of anti-p38 MAPK (IP:80821-2-RR, 4ug; Detection:80821-2-RR 1:2000) with HEK-293 cells lysate 1575 ug. This data was developed using the same antibody clone with 80821-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 80821-2-RR (same clone as 80821-2-PBS)

    IF Staining of HeLa using 80821-2-RR (same clone as 80821-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using p38 MAPK antibody (80821-2-RR, Clone: 230263D11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80821-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 80821-2-RR (same clone as 80821-2-PBS)

    FC experiment of HeLa using 80821-2-RR (same clone as 80821-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human p38 MAPK (80821-2-RR, Clone:230263D11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 80821-2-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 80821-2-RR (same clone as 80821-2-PBS)

    FC experiment of A431 using 80821-2-RR (same clone as 80821-2-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human p38 MAPK (80821-2-RR, Clone:230263D11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 80821-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80821-2-RR

    Affinity and Kinetic Characterization of 80821-2-RR

    Biolayer interferometry (BLl) kinetic assays of 80821-2-RR against Human p38 MAPK were performed. The affinity constant is 0.435 nM.

p38 MAPK Recombinant antibody, PBS Only

p38 MAPK Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 80821-2-PBS

产品说明书

CloneNo. 230263D11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

MAPK14, CSPB1, CSBP2, CSBP1, CSBP

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy p38 MAPK Ready-To-Use IHC Kit. p38 MAPK primary antibody included.

产品信息

80821-2-PBS targets p38 MAPK in WB, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 p38 MAPK fusion protein Ag5115 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mitogen-activated protein kinase 14
别名 MAPK14, CSPB1, CSBP2, CSBP1, CSBP
计算分子量 360 aa, 41 kDa
观测分子量38-42 kDa
GenBank蛋白编号BC031574
基因名称 p38 MAPK
Gene ID (NCBI) 1432
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16539
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAPK14(mitogen-activated protein kinase 14) is also named as SAPK2A, p38MAPK, CSBP1, RK, p38, EXIP, Mxi2, CSBP2, PRKM14, PRKM15, CSPB1, p38ALPHA and belongs to the MAP kinase subfamily. MAPK14-signaling is a central pathway for the integration of instructive signals in dendritic cells for T(H)17 differentiation and inflammation(PMID:22231518). It plays an important role in the regulation of hematopoietic stem cell self-renewal in vitro and inhibition of MAPK14 activation with a small molecule inhibitor may represent a novel approach to promote ex vivo expansion of hematopoietic stem cell(PMID:21198398). This protein has 4 isoforms produced by alternative splicing.