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p38 MAPK Recombinant antibody, PBS Only

p38 MAPK Uni-rAbTM Recombinant Antibody for Indirect ELISA, IP, WB

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

Indirect ELISA, IP, WB

Conjugate

Unconjugated

CloneNo.

230263H7

Cat no : 80821-3-PBS

Print datasheet

Synonyms

MAPK14, CSPB1, CSBP2, CSBP1, CSBP

验证数据展示

  • WB analysis of mouse brain using 80821-3-RR (same clone as 80821-3-PBS)

    WB analysis of mouse brain using 80821-3-RR (same clone as 80821-3-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 80821-3-RR (p38 MAPK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80821-3-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 80821-3-RR (same clone as 80821-3-PBS)

    IP experiment of HEK-293 using 80821-3-RR (same clone as 80821-3-PBS)

    IP result of anti-p38 MAPK (IP:80821-3-RR, 4ug; Detection:80821-3-RR 1:2000) with HEK-293 cells lysate 1575 ug. This data was developed using the same antibody clone with 80821-3-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 80821-3-RR (same clone as 80821-3-PBS)

    FC experiment of HeLa using 80821-3-RR (same clone as 80821-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human p38 MAPK (80821-3-RR, Clone:230263H7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 80821-3-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 80821-3-RR (same clone as 80821-3-PBS)

    FC experiment of Jurkat using 80821-3-RR (same clone as 80821-3-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Anti-Human p38 MAPK (80821-3-RR, Clone:230263H7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 80821-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80821-3-RR

    Affinity and Kinetic Characterization of 80821-3-RR

    Biolayer interferometry (BLl) kinetic assays of 80821-3-RR against Human p38 MAPK were performed. The affinity constant is 1.04 nM.

Planning an IHC experiment? We recommend our IHCeasy p38 MAPK Ready-To-Use IHC Kit. p38 MAPK primary antibody included.

产品信息

80821-3-PBS targets p38 MAPK in Indirect ELISA, IP, WB applications and shows reactivity with mouse, human samples.

Tested Applications Indirect ELISA, IP, WB Application Description
Tested Reactivity mouse, human
Immunogen p38 MAPK fusion protein Ag5115 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mitogen-activated protein kinase 14
Synonyms MAPK14, CSPB1, CSBP2, CSBP1, CSBP
Calculated Molecular Weight 360 aa, 41 kDa
Observed Molecular Weight38-42 kDa
GenBank Accession NumberBC031574
Gene Symbol p38 MAPK
Gene ID (NCBI) 1432
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16539
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAPK14(mitogen-activated protein kinase 14) is also named as SAPK2A, p38MAPK, CSBP1, RK, p38, EXIP, Mxi2, CSBP2, PRKM14, PRKM15, CSPB1, p38ALPHA and belongs to the MAP kinase subfamily. MAPK14-signaling is a central pathway for the integration of instructive signals in dendritic cells for T(H)17 differentiation and inflammation(PMID:22231518). It plays an important role in the regulation of hematopoietic stem cell self-renewal in vitro and inhibition of MAPK14 activation with a small molecule inhibitor may represent a novel approach to promote ex vivo expansion of hematopoietic stem cell(PMID:21198398). This protein has 4 isoforms produced by alternative splicing.