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验证数据展示

  • WB analysis of mouse brain using 80821-3-RR

    WB analysis of mouse brain using 80821-3-RR

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 80821-3-RR (p38 MAPK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 80821-3-RR

    IP experiment of HEK-293 using 80821-3-RR

    IP result of anti-p38 MAPK (IP:80821-3-RR, 4ug; Detection:80821-3-RR 1:2000) with HEK-293 cells lysate 1575 ug.

  • FC experiment of HeLa using 80821-3-RR

    FC experiment of HeLa using 80821-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human p38 MAPK (80821-3-RR, Clone:230263H7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • FC experiment of Jurkat using 80821-3-RR

    FC experiment of Jurkat using 80821-3-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Anti-Human p38 MAPK (80821-3-RR, Clone:230263H7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 80821-3-RR

    Affinity and Kinetic Characterization of 80821-3-RR

    Biolayer interferometry (BLl) kinetic assays of 80821-3-RR against Human p38 MAPK were performed. The affinity constant is 1.04 nM.

p38 MAPK Recombinant antibody

p38 MAPK Uni-rAbTM Recombinant Antibody for WB, FC (Intra), IP, ELISA
Cat No. 80821-3-RR

产品说明书

CloneNo. 230263H7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), IP, ELISA

别名

MAPK14, CSPB1, CSBP2, CSBP1, CSBP

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IP detected inHEK-293 cells
Positive FC (Intra) detected inHeLa cells, Jurkat cells
Planning an IHC experiment? We recommend our IHCeasy p38 MAPK Ready-To-Use IHC Kit. p38 MAPK primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80821-3-RR targets p38 MAPK in WB, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 p38 MAPK fusion protein Ag5115 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mitogen-activated protein kinase 14
别名 MAPK14, CSPB1, CSBP2, CSBP1, CSBP
计算分子量 360 aa, 41 kDa
观测分子量38-42 kDa
GenBank蛋白编号BC031574
基因名称 p38 MAPK
Gene ID (NCBI) 1432
RRIDAB_3670490
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16539
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAPK14(mitogen-activated protein kinase 14) is also named as SAPK2A, p38MAPK, CSBP1, RK, p38, EXIP, Mxi2, CSBP2, PRKM14, PRKM15, CSPB1, p38ALPHA and belongs to the MAP kinase subfamily. MAPK14-signaling is a central pathway for the integration of instructive signals in dendritic cells for T(H)17 differentiation and inflammation(PMID:22231518). It plays an important role in the regulation of hematopoietic stem cell self-renewal in vitro and inhibition of MAPK14 activation with a small molecule inhibitor may represent a novel approach to promote ex vivo expansion of hematopoietic stem cell(PMID:21198398). This protein has 4 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for p38 MAPK antibody 80821-3-RRDownload protocol
IP protocol for p38 MAPK antibody 80821-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols