CoraLite® Plus 488-conjugated p38 MAPK Recombinant antibody
p38 MAPK Uni-rAbTM Recombinant Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
230263H7
验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy p38 MAPK Ready-To-Use IHC Kit. p38 MAPK primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80821-3-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-80821-3 targets p38 MAPK in FC (Intra) applications and shows reactivity with human, mouse samples.
| Tested Applications | FC (Intra) Application Description |
| Tested Reactivity | human, mouse |
| Immunogen | p38 MAPK fusion protein Ag5115 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | mitogen-activated protein kinase 14 |
| Synonyms | MAPK14, CSPB1, CSBP2, CSBP1, CSBP |
| Calculated Molecular Weight | 360 aa, 41 kDa |
| Observed Molecular Weight | 38-42 kDa |
| GenBank Accession Number | BC031574 |
| Gene Symbol | p38 MAPK |
| Gene ID (NCBI) | 1432 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q16539 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAPK14(mitogen-activated protein kinase 14) is also named as SAPK2A, p38MAPK, CSBP1, RK, p38, EXIP, Mxi2, CSBP2, PRKM14, PRKM15, CSPB1, p38ALPHA and belongs to the MAP kinase subfamily. MAPK14-signaling is a central pathway for the integration of instructive signals in dendritic cells for T(H)17 differentiation and inflammation(PMID:22231518). It plays an important role in the regulation of hematopoietic stem cell self-renewal in vitro and inhibition of MAPK14 activation with a small molecule inhibitor may represent a novel approach to promote ex vivo expansion of hematopoietic stem cell(PMID:21198398). This protein has 4 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 p38 MAPK antibody CL488-80821-3 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
