CoraLite® Plus 488-conjugated p504S,AMACR Monoclonal antibody
p504S,AMACR Monoclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2H9B5
验证数据展示
经过测试的应用
Positive IF/ICC detected in | A431 cells |
Positive FC (Intra) detected in | LNCaP cells |
For other applications, we recommend the unconjugated version of this antibody, 60240-1-Ig
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-60240 targets p504S,AMACR in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.
Tested Applications | IF/ICC, FC (Intra) Application Description |
Tested Reactivity | human, mouse |
Immunogen | p504S,AMACR fusion protein Ag8720 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | alpha-methylacyl-CoA racemase |
Synonyms | AMACR, p504S, EC:5.1.99.4, AMACR/p504S, Alpha-methylacyl-CoA racemase |
Calculated Molecular Weight | 382 aa, 42 kDa |
Observed Molecular Weight | 42 kDa |
GenBank Accession Number | BC009471 |
Gene Symbol | AMACR |
Gene ID (NCBI) | 23600 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q9UHK6 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AMACR(Alpha-methylacyl-CoA racemase) belongs to the CaiB/BaiF CoA-transferase family. It is a mitochondrial and peroxisomal enzyme that catalyzes the conversion of 2R stereoisomers of phytanic and pristanic acid to their S counterparts. AMACR has previously been shown to be a highly sensitive marker for colorectal and clinically localized prostate cancer (PCa). However, AMACR expression is down-regulated at the transcript and protein level in hormone-refractory metastatic PCa, suggesting a hormone-dependent expression of AMACR(PMID:12213712). It has 3 isoforms produced by alternative splicing. Defects in AMACR are the cause of alpha-methylacyl-CoA racemase deficiency (AMACRD) and congenital bile acid synthesis defect type 4 (CBAS4).
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 p504S,AMACR antibody CL488-60240 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |