验证数据展示
经过测试的应用
| Positive WB detected in | HSC-T6 cells, HCT 116 cells, HeLa cells, MDA-MB-231 cells, NIH/3T3 cells, RAW 264.7 cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy CDKN1C Ready-To-Use IHC Kit. CDKN1C primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 7 publications below |
产品信息
66794-1-Ig targets p57Kip2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | p57Kip2 fusion protein Ag19927 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | cyclin-dependent kinase inhibitor 1C (p57, Kip2) |
| 别名 | CDKN1C, KIP2, p57, Cyclin-dependent kinase inhibitor p57, Cyclin-dependent kinase inhibitor 1C |
| 计算分子量 | 316 aa, 32 kDa |
| 观测分子量 | 57 kDa |
| GenBank蛋白编号 | BC067842 |
| 基因名称 | p57 Kip2/CDKN1C |
| Gene ID (NCBI) | 1028 |
| RRID | AB_2882138 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P49918 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CDKN1C, also named as p57KIP2 and KIP2, belongs to the CDI family. CDKN1C is a potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. It is a negative regulator of cell proliferation. CDKN1C may play a role in maintenance of the non-proliferative state throughout life.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for p57Kip2 antibody 66794-1-Ig | Download protocol |
| IHC protocol for p57Kip2 antibody 66794-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun The transcription factor NF-Y participates to stem cell fate decision and regeneration in adult skeletal muscle. | ||
Toxicology Toxicity mechanism of peri-implantation pesticide beta-cypermethrin exposure on endometrial remodeling in early pregnant mice | ||
J Transl Med STK40 inhibits trophoblast fusion by mediating COP1 ubiquitination to degrade P57Kip2 | ||
bioRxiv D-type cyclins regulate DNA mismatch repair in the G1 and S phases of the cell cycle, maintaining genome stability | ||
Environ Toxicol Melatonin promotes cell cycle progression of neural stem cells subjected to manganese via Nurr1 |
