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- KD/KO Validated
p70(S6K) Polyclonal antibody
p70(S6K) Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (3)
Applications
FC, IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
145
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, IGF-1 treated MCF-7 cells, Jurkat cells, NIH/3T3 cells, HeLa cells, PC-12 cells, C6 cells, mouse brain tissue, rat brain tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14485-1-AP targets p70(S6K) in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC, IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, rabbit, pig, Megalobrama amblycephala |
Immunogen | p70(S6K) fusion protein Ag5883 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ribosomal protein S6 kinase, 70kDa, polypeptide 1 |
Synonyms | p70 alpha, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6K 1, p70 S6K alpha, p70 S6KA, p70(S6K), p70(S6K) alpha, p70S6K, P70S6K1, PS6K, Ribosomal protein S6 kinase I, RPS6KB1, S6K, S6K beta 1, S6K1, STK14A |
Calculated Molecular Weight | 59 kDa |
Observed Molecular Weight | 65-75 kDa, 80-85 kDa |
GenBank Accession Number | BC053365 |
Gene Symbol | RPS6KB1 |
Gene ID (NCBI) | 6198 |
RRID | AB_2269787 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P23443 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for p70(S6K) antibody 14485-1-AP | Download protocol |
IHC protocol for p70(S6K) antibody 14485-1-AP | Download protocol |
IF protocol for p70(S6K) antibody 14485-1-AP | Download protocol |
IP protocol for p70(S6K) antibody 14485-1-AP | Download protocol |
FC protocol for p70(S6K) antibody 14485-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Stem Cell Amino acid catabolism regulates hematopoietic stem cell proteostasis via a GCN2-eIF2α axis. | ||
Small Methods PROTAC Degraders of Androgen Receptor-Integrated Dissolving Microneedles for Androgenetic Alopecia and Recrudescence Treatment via Single Topical Administration | ||
Cell Death Differ Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration | ||
Redox Biol Aberrant hyper-expression of the RNA binding protein GIGYF2 in endothelial cells modulates vascular aging and function | ||
Autophagy SMCR8 negatively regulates AKT and MTORC1 signaling to modulate lysosome biogenesis and tissue homeostasis. |