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验证数据展示

  • FC experiment of HeLa using CL647-14485

    FC experiment of HeLa using CL647-14485

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human p70(S6K) (CL647-14485) (red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated p70(S6K) Polyclonal antibody

p70(S6K) Polyclonal Antibody for FC (Intra)
Cat No. CL647-14485

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

Phospho-p70(S6K) (Thr389), RPS6KB1, p70 S6 kinase, p70 S6K, p70(S6K), p70S6K, p70-S6K 1, P70S6K1, p70 S6K 1, p70 S6K-alpha, S6K, S6K-beta-1, Serine/threonine-protein kinase 14A, Ribosomal protein S6 kinase beta-1, Ribosomal protein S6 kinase I, RPS6KB 1, 70 kDa ribosomal protein S6 kinase 1, EC:2.7.11.1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RPS6KB1 Ready-To-Use IHC Kit. RPS6KB1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14485-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-14485 targets p70(S6K) in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 p70(S6K) fusion protein Ag5883 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal protein S6 kinase, 70kDa, polypeptide 1
别名 Phospho-p70(S6K) (Thr389), RPS6KB1, p70 S6 kinase, p70 S6K, p70(S6K), p70S6K, p70-S6K 1, P70S6K1, p70 S6K 1, p70 S6K-alpha, S6K, S6K-beta-1, Serine/threonine-protein kinase 14A, Ribosomal protein S6 kinase beta-1, Ribosomal protein S6 kinase I, RPS6KB 1, 70 kDa ribosomal protein S6 kinase 1, EC:2.7.11.1
计算分子量 59 kDa
观测分子量60-75 kDa
GenBank蛋白编号BC053365
基因名称 p70 S6K
Gene ID (NCBI) 6198
RRIDAB_2934896
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP23443
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPS6KB1(Ribosomal protein S6 kinase beta-1) is also named as STK14A, p70 S6KA and belongs to the S6 kinase subfamily. RPS6KB1 is a major substrate of MTOR and acts as a crucial effector of MTOR signaling pathway. It plays a key role in cell growth and proliferation by regulating INS sensitivity, metabolism, protein synthesis, and cell cycle. RPS6KB1 may play an important role in the progression of HCC and could serve as a potential molecular target for HCC therapy (PMID:22684641). RPS6KB1 is a 70 kDa protein and has 5 isoforms with the calculated molecular mass of 51-59 kDa produced by alternative initiation.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 p70(S6K) antibody CL647-14485Download protocol
Standard Protocols
Click here to view our Standard Protocols