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pan-RAS Recombinant antibody, PBS Only (Detector)

pan-RAS Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2I22

Cat no : 81615-1-PBS

Print datasheet

Synonyms

KRAS, KRAS4A, KRAS4B, pan RAS, EC:3.6.5.2

验证数据展示

  • WB analysis using 81615-1-RR (same clone as 81615-1-PBS)

    WB analysis using 81615-1-RR (same clone as 81615-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81615-1-RR (pan-RAS antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81615-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 81615-1-RR (same clone as 81615-1-PBS)

    IHC staining of human colon cancer using 81615-1-RR (same clone as 81615-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81615-1-RR (pan-RAS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81615-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 81615-1-RR (same clone as 81615-1-PBS)

    IHC staining of human colon cancer using 81615-1-RR (same clone as 81615-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81615-1-RR (pan-RAS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81615-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 81615-1-RR (same clone as 81615-1-PBS)

    IF Staining of HeLa using 81615-1-RR (same clone as 81615-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using pan-RAS antibody (81615-1-RR, Clone: 2I22 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81615-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP80009-1

    Cytometric bead array standard curve of MP80009-1

    Cytometric bead array standard curve of MP80009-1, KRAS Hybrid Matched Antibody Pair, PBS Only. Capture antibody: 60309-1-PBS. Detection antibody: 81615-1-PBS. Standard: Ag2700. Range: 0.391-50 ng/mL.

  • Affinity and Kinetic Characterization of 81615-1-PBS

    Affinity and Kinetic Characterization of 81615-1-PBS

    Biolayer interferometry (BLl) kinetic assay of 81615-1-PBS against Human pan-RAS was performed. The affinity constant is 2.45 nM.

  • Affinity and Kinetic Characterization of 81615-1-RR

    Affinity and Kinetic Characterization of 81615-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81615-1-RR against Human pan-RAS were performed. The affinity constant is below 1 pM.

产品信息

81615-1-PBS targets pan-RAS as part of a matched antibody pair:

MP80009-1: 60309-1-PBS capture and 81615-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen pan-RAS fusion protein Ag2700 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Synonyms KRAS, KRAS4A, KRAS4B, pan RAS, EC:3.6.5.2
Calculated Molecular Weight 188 aa, 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC013572
Gene Symbol KRAS
Gene ID (NCBI) 3845
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01116
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) belong to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. K-Ras, H-Ras, and N-Ras have similar structure and sequences. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. The ras genes are ubiquitously expressed although mRNA analysis suggests different level expression in tissue. Mutations in each ras gene frequently were found in different tumors, suggesting their involvement in the development of specific neoplasia. This antibody can recognize K-Ras, H-Ras, and N-Ras.