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验证数据展示

  • WB analysis using 81615-1-RR (same clone as 81615-1-PBS)

    WB analysis using 81615-1-RR (same clone as 81615-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81615-1-RR (pan-RAS antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81615-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 81615-1-RR (same clone as 81615-1-PBS)

    IHC staining of human colon cancer using 81615-1-RR (same clone as 81615-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81615-1-RR (pan-RAS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81615-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 81615-1-RR (same clone as 81615-1-PBS)

    IHC staining of human colon cancer using 81615-1-RR (same clone as 81615-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81615-1-RR (pan-RAS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81615-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 81615-1-RR (same clone as 81615-1-PBS)

    IF Staining of HeLa using 81615-1-RR (same clone as 81615-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using pan-RAS antibody (81615-1-RR, Clone: 2I22 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81615-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP80009-1

    Cytometric bead array standard curve of MP80009-1

    Cytometric bead array standard curve of MP80009-1, KRAS Hybrid Matched Antibody Pair, PBS Only. Capture antibody: 60309-1-PBS. Detection antibody: 81615-1-PBS. Standard: Ag2700. Range: 0.391-50 ng/mL.

  • Affinity and Kinetic Characterization of 81615-1-PBS

    Affinity and Kinetic Characterization of 81615-1-PBS

    Biolayer interferometry (BLl) kinetic assay of 81615-1-PBS against Human pan-RAS was performed. The affinity constant is 2.45 nM.

  • Affinity and Kinetic Characterization of 81615-1-RR

    Affinity and Kinetic Characterization of 81615-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81615-1-RR against Human pan-RAS were performed. The affinity constant is below 1 pM.

pan-RAS Recombinant antibody, PBS Only (Detector)

pan-RAS Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 81615-1-PBS

产品说明书

CloneNo. 2I22

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

KRAS, pan RAS, EC:3.6.5.2, c-K-ras, c-Ki-ras

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

81615-1-PBS targets pan-RAS as part of a matched antibody pair:

MP80009-1: 60309-1-PBS capture and 81615-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 pan-RAS fusion protein Ag2700 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
别名 KRAS, pan RAS, EC:3.6.5.2, c-K-ras, c-Ki-ras
计算分子量 188 aa, 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC013572
基因名称 KRAS
Gene ID (NCBI) 3845
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP01116
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) belong to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. K-Ras, H-Ras, and N-Ras have similar structure and sequences. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. The ras genes are ubiquitously expressed although mRNA analysis suggests different level expression in tissue. Mutations in each ras gene frequently were found in different tumors, suggesting their involvement in the development of specific neoplasia. This antibody can recognize K-Ras, H-Ras, and N-Ras.