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验证数据展示

  • IF Staining of MCF-7 using CL594-60202

    IF Staining of MCF-7 using CL594-60202

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®594 peroxiredoxin 2 antibody (CL594-60202, Clone: 3H6C4 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated peroxiredoxin 2 Monoclonal antibody

peroxiredoxin 2 Monoclonal Antibody for IF/ICC
Cat No. CL594-60202

产品说明书

CloneNo. 3H6C4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

IF/ICC

别名

NKEF B, NKEFB, peroxiredoxin 2, PRDX2, PRP, PRX2, PRXII, TDPX1, Thioredoxin peroxidase 1, TSA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 60202-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60202 targets peroxiredoxin 2 in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原 peroxiredoxin 2 fusion protein Ag0835 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 peroxiredoxin 2
别名 NKEF B, NKEFB, peroxiredoxin 2, PRDX2, PRP, PRX2, PRXII, TDPX1, Thioredoxin peroxidase 1, TSA
计算分子量 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC003022
基因名称 peroxiredoxin 2
Gene ID (NCBI) 7001
RRIDAB_2883440
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP32119
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRDX2, also named as TSA, PRP, NKEFB and TDPX1, belongs to the ahpC/TSA family. It is known to act as an antioxidant enzyme whose main function is H2O2 reduction in cells. PRDX2 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system. It may play an important role in eliminating peroxides generated during metabolism. PRDX2 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. PRDX2 actions may be related to the expression of NFKB and IKB.(PMID:21248284)

实验方案

Product Specific Protocols
IF protocol for CL594 peroxiredoxin 2 antibody CL594-60202Download protocol
Standard Protocols
Click here to view our Standard Protocols