• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Phospho-RCC1 (Ser11) Recombinant antibody, PBS Only

Phospho-RCC1 (Ser11) Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230056B10

Cat no : 83630-1-PBS

Print datasheet

Synonyms

phospho-RCC1(Ser11), RCC1, RCC1 I, CHC1, 230056B10

验证数据展示

  • WB analysis using 83630-1-RR (same clone as 83630-1-PBS)

    WB analysis using 83630-1-RR (same clone as 83630-1-PBS)

    Non-treated and nocodazole treated HeLa cells were subjected to SDS PAGE followed by western blot with 83630-1-RR (phospho-RCC1(Ser11) antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) subsequently. This data was developed using the same antibody clone with 83630-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy RCC1 Ready-To-Use IHC Kit. RCC1 primary antibody included.

产品信息

83630-1-PBS targets Phospho-RCC1 (Ser11) in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name regulator of chromosome condensation 1
Synonyms phospho-RCC1(Ser11), RCC1, RCC1 I, CHC1, 230056B10
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberNM_001048194
Gene Symbol RCC1
Gene ID (NCBI) 1104
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP18754
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CHC1, also named as RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation. This antibody is a rabbit polyclonal antibody raised against human phospho-CHC1(Ser11) antigen.