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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human G-CSF protein- GMP grade

EC50

0.009-0.05 ng/mL

Specific Activity

minimally 1.00 x 108 IU/mg, typically 3.24 x 108 IU/mg

Species Reactivity

human,mouse

Purity

>95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat No : HZ-1207-GMP

Print datasheet

Synonyms

C17orf33, CSF3, Filgrastim, G CSF, GCSF, G-CSF, Lenograstim, Pluripoietin

Validation Data Gallery

  • Purity of GMP-grade recombinant human G-CSF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of GMP-grade recombinant human G-CSF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • GMP-grade recombinant human G-CSF (HZ-1207-GMP) Stimulates dose-dependent proliferation of the M-NFS-60 Mouse Myeloid Leukemia indicator cells line. Viable cell number was quantitatively assessed by PrestoBlue Cell Viability Reagent. M-NFS-60 cells were treated with increasing concentrations of recombinant human G-CSF for 72 hours. The EC50 was determined using a 4- parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 0.009-0.05 ng/mL.

    GMP-grade recombinant human G-CSF (HZ-1207-GMP) Stimulates dose-dependent proliferation of the M-NFS-60 Mouse Myeloid Leukemia indicator cells line. Viable cell number was quantitatively assessed by PrestoBlue Cell Viability Reagent. M-NFS-60 cells were treated with increasing concentrations of recombinant human G-CSF for 72 hours. The EC50 was determined using a 4- parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 0.009-0.05 ng/mL.

Technical Specifications

GeneID 1440
Species Human
Expression HEK293
EC50 0.009-0.05 ng/mL
Specific Activityminimally 1.00 x 108 IU/mg, typically 3.24 x 108 IU/mg
Purity >95%
Endotoxin<0.1 EU/μg
Accession NumberP09919
Molecular Mass 21 to 25 kDa reduced and non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

Granulocyte colony-stimulating factor (G-CSF), also known as CSF3, is a protective cytokine with anti-inflammatory effects. G-CSF is important in promoting survival of the granulocytic lineage cells and proliferation and migration of neutrophils and trophoblast cells. G-CSF exerts its effects by binding to its receptor G-CSFR, in turn activating the JAK/STAT and Ras/Raf/MAP kinase pathways (PMID 9015216, 7509213, 9933603). G-CSF potently stimulates the proliferation and release of peripheral blood progenitor cells into the bloodstream and is therefore used to treat neutropenia after chemotherapy (PMID 7524753, 3264199). Furthermore, G-CSF levels are elevated upon intensive exercise leading to increased neutrophil counts, which are predominantly due to delayed neutrophil apoptosis (PMID 18524991). GMP grade Humankine® Recombinant Human G-CSF was expressed and purified tag free from HEK293 human cell line without the use of any animal components.

Synonyms

C17orf33, CSF3, Filgrastim, G CSF, GCSF, G-CSF, Lenograstim, Pluripoietin