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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-23 protein- GMP grade

EC50

0.2-1.2 ng/mL in HEK293 reporter cell line

Species Reactivity

human,mouse

Purity

>95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat No : HZ-1254-GMP

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Synonyms

IL 23, IL 23 A, IL 23 subunit alpha, IL 23A, IL 23p19, IL23, IL-23, IL23A, IL23P19, Interleukin 23 subunit alpha, Interleukin 23 subunit p19, P19, SGRF

Validation Data Gallery

  • Recombinant human GMP IL-23 (HZ-1254-GMP) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 0.2-1.2 ng/mL.

    Recombinant human GMP IL-23 (HZ-1254-GMP) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 0.2-1.2 ng/mL.

Technical Specifications

GeneID 51561,3593
Species Human
Expression HEK293
EC50 0.2-1.2 ng/mL in HEK293 reporter cell line
Purity >95%
Endotoxin< 0.1 EU/µg
Accession NumberQ9NPF7
Molecular Mass 20 and 42 kDa reduced, 53 kDa non-reduced, heterodimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

Interleukin 23 (IL-23) is a member of the IL12 cytokine family and composed of two subunits, IL12p40 and IL23p19. It is produced by antigen presenting cells and has been shown to promote the production and survival of a distinct lineage of T-cells called TH17 cells. A functional receptor for IL-23 (the IL-23 receptor) has been identified and is composed of IL-12Rβ1 and IL-23R. IL-23 is expressed chiefly by the macrophages and DCs. The IL-23R is found on memory T cells, NKT cells, macrophages, DCs, and naive T cells upon activation by TGF-βand IL-6. The main biological effects of IL-23 identified initially are stimulation of antigen presentation by DCs, T cell differentiation to Th17 cells, and production of interferon-γ (IFN-γ). IL-23 acts also as an end-stage effector cytokine through direct action on macrophages (PMID: 16393998;15657292;12023369;12610626)

Synonyms

IL 23, IL 23 A, IL 23 subunit alpha, IL 23A, IL 23p19, IL23, IL-23, IL23A, IL23P19, Interleukin 23 subunit alpha, Interleukin 23 subunit p19, P19, SGRF