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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-34 protein

Activity

Active

Species Reactivity

human

Purity

>95%

Cat No : HZ-1316

Print datasheet

Synonyms

IL34, Interleukin-34, C16orf77, MGC34647

Validation Data Gallery

  • Purity of recombinant human IL-34 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

    Purity of recombinant human IL-34 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

  • 1 million THP-1 cells were seeded in a 24-well plate with serum free RPMI 1640 medium overnight. ​ Cells were treated with indicated amounts of IL-34 (HZ-1316) for 5 min before lysis with RIPA buffer. ​ Phosphorylation ERK 1/2 (PTG #28733-1-AP) and total ERK 1/2 (PTG #11257-1-AP) antibodies were​ used to detect IL-34 induced ERK1/2 phosphorylation.

    1 million THP-1 cells were seeded in a 24-well plate with serum free RPMI 1640 medium overnight. ​ Cells were treated with indicated amounts of IL-34 (HZ-1316) for 5 min before lysis with RIPA buffer. ​ Phosphorylation ERK 1/2 (PTG #28733-1-AP) and total ERK 1/2 (PTG #11257-1-AP) antibodies were​ used to detect IL-34 induced ERK1/2 phosphorylation.

Technical Specifications

GeneID 146433
Species Human
Expression HEK293
Activity Active
Purity >95%
Endotoxin<1 EU/µg
Accession NumberQ6ZMJ4
Molecular Mass 68 and 38 kDa
Formulation 50mM Acetate pH 4.5 + 75mM NaCl, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 50mM Acetate pH 4.5.

Background

IL-34 is a 6-α-helix, proinflammatory cytokine that serves as a ligand for colony-stimulating factor-1 receptor (CSF1R). It is expressed most abundantly by spleen cells. It primarily stimulates human peripheral blood monocyte proliferation and macrophage colony formation. It is also required for Langerhans cell and microglia development. IL-34 has been shown to play a protective role in cancer and Alzheimer’s disease, but it can promote the progression of several autoimmune, inflammatory, and metabolic diseases. As such IL-34 could be used as a biomarker to mark the progression of these diseases (PMID: 22579672, 27577879, 31940023).

Synonyms

IL34, Interleukin-34, C16orf77, MGC34647