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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human R-Spondin-1 protein- GMP grade

EC50

4-20 ng/mL

Species Reactivity

human

Purity

>95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat No : HZ-1328-GMP

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Synonyms

Roof-plate specific spondin-1, Critsin3, RSPO1,HRspo1

Validation Data Gallery

  • Purity of recombinant human R-Spondin-1 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human R-Spondin-1 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • GMP Recombinant human R-Spondin-1 (HZ-1328-GMP) induces dose-dependent luciferase production in a HEK293 TCF/LEF reporter cell line. Luciferase production was assessed by One-Step™ luciferase assay Kit. HEK293 TCF/LEF reporter cells were treated with increasing concentrations of recombinant R-Spondin-1 for 6 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 Range is 4-20 ng/mL.

    GMP Recombinant human R-Spondin-1 (HZ-1328-GMP) induces dose-dependent luciferase production in a HEK293 TCF/LEF reporter cell line. Luciferase production was assessed by One-Step™ luciferase assay Kit. HEK293 TCF/LEF reporter cells were treated with increasing concentrations of recombinant R-Spondin-1 for 6 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 Range is 4-20 ng/mL.

Technical Specifications

GeneID 284654
Species Human
Expression HEK293
EC50 4-20 ng/mL
Purity >95%
Endotoxin<0.1 EU/μg
Accession Number2QMKA7
Molecular Mass 36-50 kDa reduced and non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

R-Spondin 1 is an approximately 40kDA, glycosylated protein that serves as a major agonist of the Wnt signaling pathway. It regulates the turnover of the LRP6 co-receptor by preventing its internalization by DKK-1. R-Spondin 1 plays several roles in embryonic development including sex phenotype reversal, female sex determination, and ovarian differentiation. It also promotes stem cell turnover in the mammary glands, intestine, colon, and kidneys. Increased R-Spondin 1 expression has been linked to growth and migration of ovarian cancer cells as well as decreased sensitivity of gliomas to radiation-based therapies (PMID: 17804805, 22439850, 32749219, 30572097).

Synonyms

Roof-plate specific spondin-1, Critsin3, RSPO1,HRspo1