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transgelin/SM22 Monoclonal antibody, PBS Only

transgelin/SM22 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, mouse, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A10C2

Cat No : 60213-1-PBS

Print datasheet

Synonyms

22 kDa actin binding protein, DKFZp686P11128, Protein WS3 10, SM22, SM22 alpha, SM22-a, SM22alpha, SMCC, Smooth muscle protein 22 alpha, TAGLN, TAGLN1, transgelin, TRANSGELIN 1, transgelin/SM22, transgelin/SM22 alpha, WS3 10

验证数据展示

  • WB analysis using 60213-1-Ig (same clone as 60213-1-PBS)

    WB analysis using 60213-1-Ig (same clone as 60213-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

  • WB analysis of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

    WB analysis of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

    human colon tissue were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

    IHC staining of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TAGLN antibody included
  • IHC staining of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

    IHC staining of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TAGLN antibody included
  • IF Staining of mouse heart using 60213-1-Ig (same clone as 60213-1-PBS)

    IF Staining of mouse heart using 60213-1-Ig (same clone as 60213-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using transgelin/SM22 antibody (60213-1-Ig, Clone: 2A10C2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

  • WB analysis using 60213-1-Ig (same clone as 60213-1-PBS)

    WB analysis using 60213-1-Ig (same clone as 60213-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy TAGLN Ready-To-Use IHC Kit. TAGLN primary antibody included.

产品信息

60213-1-PBS targets transgelin/SM22 in WB, IF, IHC, ELISA applications and shows reactivity with human, rat, pig, mouse, mouse samples.

Tested Applications WB, IF, IHC, ELISA Application Description
Tested Reactivity human, rat, pig, mouse, mouse
Immunogen transgelin/SM22 fusion protein Ag0764 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name transgelin
Synonyms 22 kDa actin binding protein, DKFZp686P11128, Protein WS3 10, SM22, SM22 alpha, SM22-a, SM22alpha, SMCC, Smooth muscle protein 22 alpha, TAGLN, TAGLN1, transgelin, TRANSGELIN 1, transgelin/SM22, transgelin/SM22 alpha, WS3 10
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 18+22 kDa
GenBank Accession NumberBC004927
Gene Symbol TAGLN
Gene ID (NCBI) 6876
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ01995
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells.