验证数据展示
经过测试的应用
| Positive WB detected in | pig colon tissue, human colon tissue, pig stomach tissue, human stomach tissue, rat colon tissue, rat stomach tissue, rabbit stomach tissue, mouse colon tissue |
| Positive IHC detected in | human colon tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:400 |
| Immunofluorescence (IF)-P | IF-P : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60213-1-Ig targets transgelin/SM22 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat, pig, mouse, mouse samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, rat, pig, mouse, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | transgelin/SM22 fusion protein Ag0764 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | transgelin |
| 别名 | 22 kDa actin binding protein, SM22, SM22 alpha, SM22a, SM22-a, SM22alpha, SMCC, Smooth muscle protein 22 alpha, TAGLN, TAGLN1, transgelin, transgelin/SM22, transgelin/SM22 alpha |
| 计算分子量 | 22 kDa |
| 观测分子量 | 18+22 kDa |
| GenBank蛋白编号 | BC004927 |
| 基因名称 | transgelin/SM22 |
| Gene ID (NCBI) | 6876 |
| RRID | AB_11043177 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q01995 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for transgelin/SM22 antibody 60213-1-Ig | Download protocol |
| IHC protocol for transgelin/SM22 antibody 60213-1-Ig | Download protocol |
| IF protocol for transgelin/SM22 antibody 60213-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Dev Cell Endothelial progenitor cells control remodeling of uterine spiral arteries for the establishment of utero-placental circulation | ||
Pharmacol Res LDHA mediated degradation of extracellular matrix is a potential target for the treatment of aortic dissection. | ||
Front Cell Dev Biol S-propargyl-cysteine promotes the stability of atherosclerotic plaque via maintaining vascular muscle contractile phenotype | ||
Front Cell Dev Biol Platelet-Derived Microvesicles Promote VSMC Dedifferentiation After Intimal Injury via Src/Lamtor1/mTORC1 Signaling | ||
Biochim Biophys Acta Mol Basis Dis Methamphetamine induces thoracic aortic aneurysm/dissection through C/EBPβ. |
