transgelin/SM22 Monoclonal antibody

transgelin/SM22 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, mouse, mouse

Applications

IF, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

2A10C2

Cat No : 60213-1-Ig

Print datasheet

Synonyms

22 kDa actin binding protein, SM22, SM22 alpha, SM22a, SM22-a, SM22alpha, SMCC, Smooth muscle protein 22 alpha, TAGLN, TAGLN1, transgelin, transgelin/SM22, transgelin/SM22 alpha



经过测试的应用

Positive WB detected inpig colon tissue, human colon tissue, pig stomach tissue, human stomach tissue, rat colon tissue, rat stomach tissue, rabbit stomach tissue, mouse colon tissue
Positive IHC detected inhuman colon tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue
Planning an IHC experiment? We recommend our IHCeasy TAGLN Ready-To-Use IHC Kit. TAGLN primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:400
Immunofluorescence (IF)IF : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60213-1-Ig targets transgelin/SM22 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, rat, pig, mouse, mouse samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications FC, IF, WB
Tested Reactivity human, rat, pig, mouse, mouse
Cited Reactivityhuman, mouse, rat
Immunogen transgelin/SM22 fusion protein Ag0764 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name transgelin
Synonyms 22 kDa actin binding protein, SM22, SM22 alpha, SM22a, SM22-a, SM22alpha, SMCC, Smooth muscle protein 22 alpha, TAGLN, TAGLN1, transgelin, transgelin/SM22, transgelin/SM22 alpha
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 18+22 kDa
GenBank Accession NumberBC004927
Gene Symbol TAGLN
Gene ID (NCBI) 6876
RRIDAB_11043177
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ01995
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells.

实验方案

Product Specific Protocols
WB protocol for transgelin/SM22 antibody 60213-1-IgDownload protocol
IHC protocol for transgelin/SM22 antibody 60213-1-IgDownload protocol
IF protocol for transgelin/SM22 antibody 60213-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Pharmacol Res

LDHA mediated degradation of extracellular matrix is a potential target for the treatment of aortic dissection.

Authors - Xiaohui Wu
mouseWB

Front Cell Dev Biol

S-propargyl-cysteine promotes the stability of atherosclerotic plaque via maintaining vascular muscle contractile phenotype

Authors - Li Ping
humanWB

Oncotarget

Specific role of RhoC in tumor invasion and metastasis.

Authors - Sarah Lang
mouseWB

Front Cell Dev Biol

Platelet-Derived Microvesicles Promote VSMC Dedifferentiation After Intimal Injury via Src/Lamtor1/mTORC1 Signaling

Authors - Ji-Ting Liu
ratIF

Biochim Biophys Acta Mol Basis Dis

Methamphetamine induces thoracic aortic aneurysm/dissection through C/EBPβ.

Authors - Bao-Ying Luo
human,mouseWB,IF

Front Cardiovasc Med

Insulin Resistance Promotes the Formation of Aortic Dissection by Inducing the Phenotypic Switch of Vascular Smooth Muscle Cells.

Authors - Hui Zheng