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验证数据展示

  • WB analysis using 82953-2-RR (same clone as 82953-2-PBS)

    WB analysis using 82953-2-RR (same clone as 82953-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82953-2-RR (uPAR/CD87 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82953-2-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 82953-2-RR (same clone as 82953-2-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 82953-2-RR (same clone as 82953-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82953-2-RR (uPAR/CD87 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82953-2-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 82953-2-RR (same clone as 82953-2-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 82953-2-RR (same clone as 82953-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82953-2-RR (uPAR/CD87 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82953-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 82953-2-RR (same clone as 82953-2-PBS)

    IHC staining of mouse spleen using 82953-2-RR (same clone as 82953-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82953-2-RR (uPAR/CD87 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82953-2-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 82953-2-RR (same clone as 82953-2-PBS)

    IHC staining of human lung cancer using 82953-2-RR (same clone as 82953-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82953-2-RR (uPAR/CD87 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82953-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00129-1

    Sandwich ELISA standard curve of MP00129-1

    Sandwich ELISA standard curve of MP00129-1, uPAR/CD87 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82953-2-PBS. Detection antibody: 82953-3-PBS. Standard: Ag28056. Range: 125-8000 pg/mL.

  • Sandwich ELISA standard curve of MP00129-2

    Sandwich ELISA standard curve of MP00129-2

    Sandwich ELISA standard curve of MP00129-2, uPAR/CD87Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82953-4-PBS. Detection antibody: 82953-2-PBS. Standard: Ag28056. Range: 125-8000 pg/mL.

  • Cytometric bead array standard curve of MP00129-2

    Cytometric bead array standard curve of MP00129-2

    Cytometric bead array standard curve of MP00129-2,uPAR/CD87 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82953-4-PBS. Detection antibody: 82953-2-PBS. Standard: Ag28056. Range: 1.25-20 ng/mL.

  • Affinity and Kinetic Characterization of 82953-2-RR

    Affinity and Kinetic Characterization of 82953-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82953-2-RR against Human uPAR, PLAUR were performed. The affinity constant is 0.527 nM.

uPAR/CD87 Recombinant antibody, PBS Only (Capture/Detector)

uPAR/CD87 Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 82953-2-PBS

产品说明书

CloneNo. 230148B10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

别名

uPAR, PLAUR, PLAUR, UPAR, U PAR, MO3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82953-2-PBS targets uPAR/CD87 as part of a matched antibody pair:

MP00129-1: 82953-2-PBS capture and 82953-3-PBS detection (validated in Sandwich ELISA)

MP00129-2: 82953-4-PBS capture and 82953-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 uPAR/CD87 fusion protein Ag28056 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 PLAUR
别名 uPAR, PLAUR, PLAUR, UPAR, U PAR, MO3
计算分子量 37 kDa
观测分子量35-50 kDa
GenBank蛋白编号BC002788
基因名称 uPAR
Gene ID (NCBI) 5329
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ03405
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

uPAR is a highly glycosylated, GPI-anchored membrane protein. In addition to the membrane-anchored form, uPAR is released from the plasma membrane by cleavage of the GPI anchor and can be found as a soluble form (suPAR). uPAR contains three homologous domains (D1-D3) of which the N-terminal one (D1) represents the uPA-binding domain. After binding to uPAR, uPA cleaves plasminogen, generating the active protease plasmin which is involved in a wide variety of physiologic and pathologic processes. In addition to regulating proteolysis, uPAR has important function in cell adhesion, migration and proliferation. Studies reveal that uPAR expression is elevated during inflammation and tissue remodeling and in many human cancers, in which it frequently indicates poor prognosis. (PMID 20027185; 12461559)