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uPAR/CD87 Recombinant antibody

uPAR/CD87 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

230148B10

Cat no : 82953-2-RR

Print datasheet

Synonyms

uPAR, PLAUR, PLAUR, UPAR, U PAR, MO3

验证数据展示

  • WB analysis using 82953-2-RR

    WB analysis using 82953-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82953-2-RR (uPAR/CD87 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 82953-2-RR

    IHC staining of human intrahepatic cholangiocarcinoma using 82953-2-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82953-2-RR (uPAR/CD87 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 82953-2-RR

    IHC staining of human intrahepatic cholangiocarcinoma using 82953-2-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82953-2-RR (uPAR/CD87 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 82953-2-RR

    IHC staining of mouse spleen using 82953-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82953-2-RR (uPAR/CD87 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82953-2-RR

    Affinity and Kinetic Characterization of 82953-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82953-2-RR against Human uPAR, PLAUR were performed. The affinity constant is 0.527 nM.

经过测试的应用

Positive WB detected inHeLa cells, DU 145 cells, THP-1 cells, Saos-2 cells, PC-3 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82953-2-RR targets uPAR/CD87 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen uPAR/CD87 fusion protein Ag28056 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name PLAUR
Synonyms uPAR, PLAUR, PLAUR, UPAR, U PAR, MO3
Calculated Molecular Weight 37 kDa
Observed Molecular Weight35-50 kDa
GenBank Accession NumberBC002788
Gene Symbol uPAR
Gene ID (NCBI) 5329
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ03405
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

uPAR is a highly glycosylated, GPI-anchored membrane protein. In addition to the membrane-anchored form, uPAR is released from the plasma membrane by cleavage of the GPI anchor and can be found as a soluble form (suPAR). uPAR contains three homologous domains (D1-D3) of which the N-terminal one (D1) represents the uPA-binding domain. After binding to uPAR, uPA cleaves plasminogen, generating the active protease plasmin which is involved in a wide variety of physiologic and pathologic processes. In addition to regulating proteolysis, uPAR has important function in cell adhesion, migration and proliferation. Studies reveal that uPAR expression is elevated during inflammation and tissue remodeling and in many human cancers, in which it frequently indicates poor prognosis. (PMID 20027185; 12461559)

实验方案

Product Specific Protocols
WB protocol for uPAR/CD87 antibody 82953-2-RRDownload protocol
IHC protocol for uPAR/CD87 antibody 82953-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols